Mouse Anti-Chicken Bu-1-Pacific Blue™

Cat. No.
0.1 mg  
Price (USD)
Clone AV20
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-Pacific Blue™
Immunogen Chicken peripheral blood lymphocytes and splenocytes
Specificity Chicken Bu-1
Alternalte Name(s) ChB6
Description Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated disulfide-linked homodimer. The monoclonal antibody AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. The monoclonal antibody AV20 does not cross react with turkey cells when analyzed by flow cytometry.
Format/Conjugate PACBLU (Pacific Blue™)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,5-7,13-26
Immunohistochemistry-Frozen Sections – Reported in literature 2-8
Immunohistochemistry-Paraffin Sections – Reported in literature 9-11
Immunocytochemistry – Reported in literature 1
Immunoprecipitation – Reported in literature 1,12
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken Bu-1-AF647 (SB Cat. No. 8395-31) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken Bu-1-AF647 (SB Cat. No. 8395-31) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).
1. Rothwell CJ, Vervelde L, Davison TF. Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol. 1996;55:225-34. (Immunogen, FC, IP, ICC)
2. O'Halloran EK, Oesterle EC. Characterization of leukocyte subtypes in chicken inner ear sensory epithelia. J Comp Neurol. 2004;475:340-60. (IHC-FS)
3. Ruby T, Whittaker C, Withers DR, Chelbi-Alix MK, Morin V, Oudin A, et al. Transcriptional profiling reveals a possible role for the timing of the inflammatory response in determining susceptibility to a viral infection. J Virol. 2006;80:9207-16. (IHC-FS)
4. Hansell C, Zhu XW, Brooks H, Sheppard M, Withanage S, Maskell D, et al. Unique features and distribution of the chicken CD83+ cell. J Immunol. 2007;179:5117-25. (IHC-FS)
5. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
6. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC, IHC-FS)
7. Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, et al. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. 2013;110:20170-5. (FC, IHC-FS)
8. Ciccone NA, Mwangi W, Ruzov A, Smith LP, Butter C, Nair V. A B-cell targeting virus disrupts potentially protective genomic methylation patterns in lymphoid tissue by increasing global 5-hydroxmethylcytosine levels. Vet Res. 2014;45:108. (IHC-FS)
9. Teixeira AR, Gomes C, Nitz N, Sousa AO, Alves RM, Guimaro MC, et al. Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism. PLoS Negl Trop Dis. 2011;5(3):e1000. (IHC-PS)
10. Teixeira AR, Nitz N, Bernal FM, Hecht MM. Parasite induced genetically driven autoimmune Chagas heart disease in the chicken model. J Vis Exp. 2012;65:e3716. (IHC-PS)
11. Korte J, Fröhlich T, Kohn M, Kaspers B, Arnold GJ, Härtle S. 2D DIGE analysis of the bursa of Fabricius reveals characteristic proteome profiles for different stages of chicken B-cell development. Proteomics. 2013;13:119-33. (IHC-PS)
12. Igyártó BZ, Nagy N, Magyar A, Oláh I. Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody. Poult Sci. 2008;87:351-5. (IP)
13. Schulte CE, von Lindern M, Steinlein P, Beug H, Wiedemann LM. MLL-ENL cooperates with SCF to transform primary avian multipotent cells. EMBO J. 2002;21:4297-306. (FC)
14. Javed MA, Frasca S Jr, Rood D, Cecchini K, Gladd M, Geary SJ, et al. Correlates of immune protection in chickens vaccinated with Mycoplasma gallisepticum strain GT5 following challenge with pathogenic M. gallisepticum strain Rlow. Infect Immun. 2005;73:5410-9. (FC)
15. Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)
16. Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek's disease virus. Viral Immunol. 2009;22:321-7. (FC)
17. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
18. Matulova M, Stepanova H, Sisak F, Havlickova H, Faldynova M, Kyrova K, et al. Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with Salmonella enteritidis. PLoS One. 2012;7(2):e32346. (FC)
19. Andersen JP, Norup LR, Dalgaard TS, Rothwell L, Kaiser P, Permin A, et al. No protection in chickens immunized by the oral or intra-muscular immunization route with Ascaridia galli soluble antigen. Avian Pathol. 2013;42:276-82. (FC)
20. Fernandes Filho T, Fávaro C Jr, Ingberman M, Beirão BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)
21. Chrząstek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken. Pol J Vet Sci. 2013;16:551-4. (FC)
22. Zechmann M, Reese S, Göbel TW. Chicken CRTAM binds nectin-like 2 ligand and is upregulated on CD8+ αβ and γδ T lymphocytes with different kinetics. PLOS One. 2013;8(12):e81942. (FC)
23. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)
24. Laniewski P, Kuczkowski M, Chrząstek K, Woźniak A, Wyszyńska A, Wieliczko A, et al. Evaluation of the immunogenicity of Campylobacter jejuni CjaA protein delivered by Salmonella enterica sv. Typhimurium strain with regulated delayed attenuation in chickens. World J Microbiol Biotechnol. 2014;30:281-92. (FC)
25. Machado PC Jr, Beirão BC, Filho TF, Lourenço MC, Joineau ML, Santin E, et al. Use of blends of organic acids and oregano extracts in feed and water of broiler chickens to control Salmonella Enteritidis persistence in the crop and ceca of experimentally infected birds. J Appl Poult Res. 2014;23:671-82. (FC)
26. Kjærup RM, Dalgaard TS, Norup LR, Bergman I, Sørensen P, Juul-Madsen HR. Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations. Immunobiology. 2014;219:263-74. (FC)
Pacific Blue™ is a trademark of Life Technologies Corporation