Mouse Anti-Chicken Monocyte/Macrophage-Alexa Fluor® 647

Cat. No.
8420-31  
Size
0.1 mg  
Price (USD)
$255.00 
Clone KUL01
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-Alexa Fluor® 647
Immunogen Chicken peripheral blood mononuclear cell leukocytes
Specificity Chicken Monocyte/Macrophage
Description The monoclonal antibody KUL01 is useful in the study of the development, distribution, function, and ontogeny of the mononuclear phagocyte system (MPS) of the chicken by exclusively recognizing the cells of the MPS. It identifies chicken monocytes and macrophages as well as interdigitating cells and activated microglia cells. This antibody does not react with B (Bu-1+) or T (CD3+) lymphocytes.
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3,4,14-21
Immunohistochemistry-Frozen Sections – Reported in literature 1-9
Immunohistochemistry-Paraffin Sections – Reported in literature 10,11
Immunohistochemistry-While Mount – Reported in literature 12
Immunocytochemistry – Reported in literature 6,7,13,14
Separation – Reported in literature 6,9,14,22,23
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Chicken peripheral blood monocytes were stained with Mouse Anti-Chicken Monocyte/Macrophage-PE (SB Cat. No. 8420-09) and Mouse Anti-Chicken CD44-FITC (SB Cat. No. 8400-02)

Chicken peripheral blood monocytes were stained with Mouse Anti-Chicken Monocyte/Macrophage-PE (SB Cat. No. 8420-09) and Mouse Anti-Chicken CD44-FITC (SB Cat. No. 8400-02)
 
References
1. Mast J, Goddeeris BM, Peeters K, Vandesande F, Berghman LR. Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01. Vet Immunol Immunopathol. 1998;61:343-57. (Immunogen, FC, IHC-FS)
2. Abdul-Careem MK, Hunter DB, Thanthrige-Don N, Haghighi HR, Lambourne MD, Sharif S. Cellular and cytokine responses associated with dinitrofluorobenzene-induced contact hypersensitivity in the chicken. Vet Immunol Immunopathol. 2008;122:275-84. (IHC-FS)
3. van Haarlam DA, van Kooten PJ, Rothwell L, Kaiser P, Vervelde L. Characterisation and expression analysis of the chicken interleukin-7 receptor alpha chain. Dev Comp Immunol. 2009;33:1018-26. (FC, IHC-FS)
4. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC, IHC-FS)
5. Levkut M, Revajová V, Lauková A, Ševčíková Z, Spišáková V, Faixová Z, et al. Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium EF55 and challenged with Salmonella Enteritidis. Res Vet Sci. 2012;93:195-201. (IHC-FS)
6. Chen YS, Shen PC, Su BS, Liu TC, Lin CC, Lee LH. Avian reovirus replication in mononuclear phagocytes in chicken footpad and spleen after footpad inoculation. Can J Vet Res. 2015;79:87-94. (IHC-FS, ICC, Sep)
7. Reddy VR, Trus I, Desmarets LM, Li Y, Theuns S, Nauwynck HJ. Productive replication of nephropathogenic infectious bronchitis virus in peripheral blood monocytic cells, a strategy for viral dissemination and kidney infection in chickens. Vet Res. 2016;47:70. (IHC-FS, ICC)
8. Schokker D, Jansman AJ, Veninga G, de Bruin N, Vastenhouw SA, de Bree FM, et al. Perturbation of microbiota in one-day old broiler chickens with antibiotic for 24 hours negatively affects intestinal immune development. BMC Genomics. 2017;18:241. (IHC-FS)
9. Wang D, Sun S, Heidari M. Marek's disease vaccine activates chicken macrophages. J Vet Sci. 2018;19:375-83. (IHC-FS, Sep)
10. Teixeira AR, Gomes C, Nitz N, Sousa AO, Alves RM, Guimaro MC, et al. Trypanosoma cruzi in the chicken model: Chagas-like heart disease in the absence of parasitism. PLoS Negl Trop Dis. 2011;5(3):e1000. (IHC-PS)
11. Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. (IHC-PS)
12. Warchol ME, Schwendener RA, Hirose K. Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea. PLoS One. 2012;7(12):e51574. (IHC-WM)
13. Chen YS, Wu HC, Shien JH, Chiu HH, Lee LH. Cloning and characterization of a 7 transmembrane receptor from the adherent cells of chicken peripheral blood mononuclear cells. PLoS One. 2014;9(1):e86880. (ICC)
14. Kraaij MD, van Dijk A, Haagsman HP. CATH-2 and LL-37 increase mannose receptor expression, antigen presentation and the endocytic capacity of chicken mononuclear phagocytes. Mol Immunol. 2017;90:118-25. (FC, ICC, Sep)
15. Viertlboeck BC, Schweinsberg S, Hanczaruk MA, Schmitt R, Du Pasquier L, Herberg FW, et al. The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor. Proc Natl Acad Sci USA. 2007;104:11718-23. (FC)
16. Del Cacho E, Gallego M, Lillehoj HS, López-Bernard F, Sánchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)
17. Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)
18. Matulova M, Stepanova H, Sisak F, Havlickova H, Faldynova M, Kyrova K, et al. Cytokine signaling in splenic leukocytes from vaccinated and non-vaccinated chickens after intravenous infection with Salmonella enteritidis. PLoS One. 2012;7(2):e32346. (FC)
19. Fernandes Filho T, Fávaro C Jr, Ingberman M, Beirão BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)
20. Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)
21. Machado PC Jr, Beirão BC, Filho TF, Lourenço MC, Joineau ML, Santin E, et al. Use of blends of organic acids and oregano extracts in feed and water of broiler chickens to control Salmonella Enteritidis persistence in the crop and ceca of experimentally infected birds. J Appl Poult Res. 2014;23:671-82. (FC)
22. Wu Z, Rothwell L, Hu T, Kaiser P. Chicken CD14, unlike mammalian CD14, is trans-membrane rather than GPI-anchored. Dev Comp Immunol. 2009;33:97-104. (Sep)
23. Rothwell L, Hu T, Wu Z, Kaiser P. Chicken interleukin-21 is costimulatory for T cells and blocks maturation of dendritic cells. Dev Comp Immunol. 2012;36:475-82. (Sep)
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