Mouse Anti-Human IgG2 Fd-Alexa Fluor® 555

Cat. No.
9080-32  
Size
0.1 mg  
Price (USD)
$262.00 
Clone HP6014
Isotype Mouse (BALB/c) IgG1κ
Immunogen Human IgG2 myeloma protein
Specificity Human IgG2 Fd
Format/Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 2-12
FLISA – Quality tested
Flow Cytometry – Reported in literature 19
Immunohistochemistry-Frozen Sections – Reported in literature 13-15
Immunohistochemistry-Paraffin Sections – Reported in literature 12,16,17
Western Blot – Reported in literature 13,18,19
Immunoprecipitation – Reported in literature 1
Purification – Reported in literature 19
Depletion – Reported in literature 20
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2895841
Gene ID
3501 (Human)
Gene Symbol
IGHG2 (Human)
Uniprot ID
P01859 (Human)
Uniprot Name
  • FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG2 Fd-AF555 (SB Cat. No. 9080-32).

FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG2 Fd-AF555 (SB Cat. No. 9080-32).
 
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References
1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen, IP)
2. Cui Z, Wang H, Zhao M. Natural autoantibodies against glomerular basement membrane exist in normal human sera. Kidney Int. 2006;69:894-9. (ELISA)
3. Gao Y, Ye H, Yu F, Guo X, Zhao M. Anti-myeloperoxidase IgG subclass distribution and avidity in sera from patients with propylthiouracil-induced antineutrophil cytoplasmic antibodies associated vasculitis. Clin Immunol. 2005;117:87-93. (ELISA)
4. Chapuy-Regaud S, Nogueira L, Clavel C, Sebbag M, Vincent C, Serre G. IgG subclass distribution of the rheumatoid arthritis-specific autoantibodies to citrullinated fibrin. Clin Exp Immunol. 2005;139:542-50. (ELISA)
5. Liu L, Chen M, Yu F, Zhao M, Wang H. IgG subclass distribution, affinity of anti-myeloperoxidase antibodies in sera from patients with Wegener's granulomatosis and microscopic polyangiitis. Nephrology. 2008;13:629-35. (ELISA)
6. Xie L, Gao Y, Li M, Lu G, Guo X. Distribution of immunoglobulin G subclasses of anti-thyroid peroxidase antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status. Clin Exp Immunol. 2008;154:172-6. (ELISA)
7. Zhao J, Yan Y, Cui Z, Yang R, Zhao M. The immunoglobulin G subclass distribution of anti-GBM autoantibodies against rHα3(IV)NC1 is associated with disease severity. Hum Immunol. 2009;70:425-9. (ELISA)
8. Xu P, Chen M, Cui Z, Zhao M. Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis. Rheumatology. 2010;49:2068-75. (ELISA)
9. Xu P, Cui Z, Chen M, Hellmark T, Zhao M. Comparison of characteristics of natural autoantibodies against myeloperoxidase and anti-myeloperoxidase autoantibodies from patients with microscopic polyangiitis. Rheumatology. 2011;50:1236-43. (ELISA)
10. Xu P, Chen M, Zhao M. High potential to reverse the inhibition of myeloperoxidase by ceruloplasmin of anti-myeloperoxidase autoantibodies of IgG3 subclass. Autoimmunity. 2012;45:218-25. (ELISA)
11. Shao C, Huo N, Zhao L, Gao Y, Fan X, Zheng Y, et al. The presence of thyroid peroxidase antibody of IgG2 subclass is a risk factor for thyroid dysfunction in chronic hepatitis C patients. Eur J Endocrinol. 2013;168:717-22. (ELISA)
12. Jia M, Hu S, Chen J, Qu Z, Liu G, Cui Z, et al. The clinical and immunological features of patients with combined anti-glomerular basement membrane disease and membranous nephropathy. Kidney Int. 2014;85:945-52. (ELISA, IHC-PS)
13. Mongan LC, Ockleford CD. Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta. J Anat. 1996;188:43-51. (IHC-FS, WB)
14. Lin J, Markowitz GS, Valeri AM, Kambham N, Sherman WH, Appel GB, et al. Renal monoclonal immunoglobulin deposition disease: the disease spectrum. J Am Soc Nephrol. 2001;12:1482-92. (IHC-FS)
15. Greenlee JE, Boyden JW, Pingree M, Brashear HR, Clawson SA, Keeney PM. Antibody types and IgG subclasses in paraneoplastic neurological syndromes. J Neurol Sci. 2001;184:131-7. (IHC-FS)
16. Qu Z, Liu G, Li J, Wu L, Tan Y, Zheng X, et al. Absence of glomerular IgG4 deposition in patients with membranous nephropathy may indicate malignancy. Nephrol Dial Transplant. 2012;27:1931-7. (IHC-PS)
17. Qu Z, Cui Z, Liu G, Zhao M. The distribution of IgG subclass deposition on renal tissues from patients with anti-glomerular basement membrane disease. BMC Immunol. 2013;14:19. (IHC-PS)
18. Harada S, Hata S, Kosada Y, Kondo E. Identification of epitopes recognized by a panel of six anti-human IgG2 monoclonal antibodies. J Immunol Methods. 1991;141:89-96. (WB)
19. Keen J, Serghides L, Ayi K, Patel SN, Ayisi J, van Eijk A, et al. HIV impairs opsonic phagocytic clearance of pregnancy-associated malaria parasites. PLoS Med. 2007;4(5):e181. (WB, FC, Purification)
20. Forthal DN, Landucci G, Ding H, Kappes JC, Wang A, Thung I, et al. IgG2 inhibits HIV-1 internalization by monocytes, and IgG subclass binding is affected by gp120 glycosylation. AIDS. 2011;25:2099-104. (Depletion)
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