Mouse Anti-Human IgA2-UNLB

Cat. No.
9140-01  
Size
0.5 mg   		Price (USD)
$150.00 
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Clone A9604D2
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Purified human IgA2m(1) myeloma protein
Specificity Human IgA2 Fc
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1-9
FLISA – Quality tested
ELISpot – Reported in literature 10
Flow Cytometry – Reported in literature 8,16
Immunohistochemistry-Frozen Sections – Reported in literature 8,11
Immunohistochemistry-Paraffin Sections – Reported in literature 12
Western Blot – Reported in literature 13-15
Multiplex – Reported in literature 17-19
Separation – Reported in literature 8
Depletion – Reported in literature 20
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796659
Gene ID
3494 (Human)
Gene Symbol
IGHA2 (Human)
Uniprot ID
P01877 (Human)
Uniprot Name
IGHA2_HUMAN 
  • Mouse Anti-Human IgA2-HRP
    ELISA plate was coated with purified human IgA1, IgA2, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA2-HRP (SB Cat. No. 9140-05).

ELISA plate was coated with purified human IgA1, IgA2, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA2-HRP (SB Cat. No. 9140-05).
 
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References
1. Johnson S, Sypura WD, Gerding DN, Ewing SL, Janoff EN. Selective neutralization of a bacterial enterotoxin by serum immunoglobulin A in response to mucosal disease. Infect Immun. 1995;63:3166-73. (ELISA)
2. Opstad NL, Daley CL, Thurn JR, Rubins JB, Merrifield C, Hopewell PC, et al. Impact of Streptococcus pneumoniae bacteremia and human immunodeficiency virus type 1 on oral mucosal immunity. J Infect Dis. 1995;172:566-70. (ELISA)
3. Islam D, Wretlind B, Ryd M, Lindberg AA, Christensson B. Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis. Infect Immun. 1995;63:2054-61. (ELISA)
4. Camacho MT, Outschoorn I, Kovácová E, Telléz A. Distribution of immunoglobulin G (IgG) and A (IgA) subclasses following Q fever vaccination with soluble phase I Coxiella burnetii extract. Vaccine. 2000;18:1773-7. (ELISA)
5. Ryan ET, Bridges EA, Crean TI, Gausia K, Hamadani JD, Aziz A, et al. Local production of anti-vibrio cholerae mucosal antibody in reproductive tract tissues after cholera. J Infect Dis. 2001;184:643-7. (ELISA)
6. Arnold M, Zacher T, Dechant M, Kalden JR, Doxiadis II, Spriewald BM. Detection and specification of noncomplement binding anti-HLA alloantibodies. Hum Immunol. 2004;65:1288-96. (ELISA)
7. Fasching CE, Grossman T, Corthésy B, Plaut AG, Weiser JN, Janoff EN. Impact of the molecular form of immunoglobulin A on functional activity in defense against Streptococcus pneumoniae. Infect Immun. 2007;75:1801-10. (ELISA)
8. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (ELISA, Sep, FC, IHC-FS)
9. Geisler WM, Morrison SG, Doemland ML, Iqbal SM, Su J, Mancevski A, et al. Immunoglobulin-specific responses to Chlamydia elementary bodies in individuals with and at risk for genital chlamydial infection. J Infect Dis. 2012;206:1836-43. (ELISA)
10. Carson PJ, Schut RL, Simpson ML, O'Brien J, Janoff EN. Antibody class and subclass responses to pneumococcal polysaccharides following immunization of human immunodeficiency virus-infected patients. J Infect Dis. 1995;172:340-5. (ELISPOT)
11. Senpuku H, Asano T, Matin K, Salam MA, Tsuha Y, Horibata S, et al. Effects of human interleukin-18 and interleukin-12 treatment on human lymphocyte engraftment in NOD-scid mouse. Immunology. 2002;107:232-42. (IHC-FS)
12. Islam D, Veress B, Bardhan PK, Lindberg AA, Christensson B. Quantitative assessment of IgG and IgA subclass producing cells in rectal mucosa during shigellosis. J Clin Pathol. 1997;50:513-20. (IHC-PS)
13. Batten MR, Senior BW, Kilian M, Woof JM. Amino acid sequence requirements in the hinge of human immunoglobulin A1 (IgA1) for cleavage by streptococcal IgA1 proteases. Infect Immun. 2003;71:1462-9. (WB)
14. Lewis MJ, Pleass RJ, Batten MR, Atkin JD, Woof JM. Structural requirements for the interaction of human IgA with the human polymeric Ig receptor. J Immunol. 2005;175:6694-701. (WB)
15. Lehoux S, Mi R, Aryal RP, Wang Y, Schjoldager KT, Clausen H, et al. Identification of distinct glycoforms of IgA1 in plasma from patients with immunoglobulin A (IgA) nephropathy and healthy individuals. Mol Cell Proteomics. 2014;13:3097-113. (WB)
16. Avery DT, Bryant VL, Ma CS, de Waal Malefyt R, Tangye SG. IL-21-induced isotype switching to IgG and IgA by human naive B cells is differentially regulated by IL-4. J Immunol. 2008;181:1767-79. (FC)
17. Arnold M, Dechant M, Doxiadis II, Spriewald BM. Prevalence and specificity of immunoglobulin G and immunoglobulin A non-complement-binding anti-HLA alloantibodies in retransplant candidates. Tissue Antigens. 2008;72:60-6. (Multiplex)
18. Arnold M, Heinemann FM, Horn P, Ziemann M, Lachmann N, Mühlbacher A, et al. 16th IHIW: anti-HLA alloantibodies of the of IgA isotype in re-transplant candidates. Int J Immunogenet. 2013;40:17-20. (Multiplex)
19. Arnold M, Ntokou I, Doxiadis II, Spriewald BM, Boletis JN, Iniotaki AG. Donor-specific HLA antibodies: evaluating the risk for graft loss in renal transplant recipients with isotype switch from complement fixing IgG1/IgG3 to noncomplement fixing IgG2/IgG4 anti-HLA alloantibodies. Transpl Int. 2014;27:253-61. (Multiplex)
20. Kitani A, Strober W. Differential regulation of Cα1 and Cα2 germ-line and mature mRNA transcripts in human peripheral blood B cells. J Immunol. 1994;153:1466-77. (Depletion)