Mouse Anti-Mouse Bax-UNLB

Cat. No.
10050-01  
Size
0.1 mg  
Price (USD)
$180.00 
Clone 5B7
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Murine Bax peptide (a.a. 3-16)
Specificity Mouse Bax
Alternalte Name(s) Apoptosis regulator BAX, BCL2 associated X protein
Description Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 5B7 recognizes both native and activated forms of Bax as well as Bax-Bcl-2 and Bax-Bcl-xL heterodimers.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Western Blot – Quality tested 1-3,5
Immunoprecipitation – Reported in literature 1,3,5
Flow Cytometry – Reported in literature 4
Immunocytochemistry – Reported in literature 4
Purification – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794106
Gene ID
12028 (Mouse)
Gene Symbol
Bax (Mouse)
Uniprot ID
Q07813 (Mouse)
Uniprot Name
Related Products (11)
References
1. Hsu Y, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997;272:13829-34. (Immunogen, WB, IP)
2. Hsu Y, Wolter KG, Youle RJ. Cytosol-to-membrane redistribution of Bax and Bcl-XL during apoptosis. Proc Natl Acad Sci USA. 1997;94:3668-72. (WB)
3. Havasi A, Li Z, Wang Z, Martin JL, Botla V, Ruchalski K, et al. Hsp27 inhibits Bax activation and apoptosis via a phosphatidylinositol 3-kinase-dependent mechanism. J Biol Chem. 2008;283:12305-13. (WB, IP)
4. Halicka HD, Smolewski P, Darzynkiewicz Z, Dai W, Traganos F. Arsenic trioxide arrests cells early in mitosis leading to apoptosis. Cell Cycle. 2002;1:200-8. (FC, ICC)
5. Hsu Y, Youle RJ. Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J Biol Chem. 1998;273:10777-83. (WB, IP, Purification)