Rat Anti-Mouse Kappa-FITC

Cat. No.
1170-02  
Size
0.5 mg  
Price (USD)
$195.00 
Clone 187.1
Isotype Rat (Sprague-Dawley) IgG1κ
Isotype Control Rat IgG1-FITC
Immunogen MPC-11 immunoglobulin
Specificity Mouse Kappa
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 4-15
ELISA – Quality tested 2,3
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 16
Western Blot-Non-Reducing – Reported in literature 4,17,18
Immunoprecipitation – Reported in literature 19
Purification – Reported in literature 20,21
Costimulation – Reported in literature 22
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794663
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse Kappa-FITC (SB Cat. No. 1170-02) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse Kappa-FITC (SB Cat. No. 1170-02) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).
 
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References
1. Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981;1:5-11. (Immunogen)
2. Förster M, Raposo B, Ekman D, Klaczkowska D, Popovic M, Nandakumar KS, et al. Genetic control of antibody production during collagen-induced arthritis development in heterogeneous stock mice. Arthritis Rheum. 2012;64:3594-603. (ELISA)
3. Raposo B, Dobritzsch D, Ge C, Ekman D, Xu B, Lindh I, et al. Epitope-specific antibody response is controlled by immunoglobulin VH polymorphisms. J Exp Med. 2014;211:405-11. (ELISA)
4. Nakamura T, Kubagawa H, Cooper MD. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci USA. 1992;89:8522-6. (FC, WB)
5. Bertocci B, De Smet A, Berek C, Weill J, Reynaud C. Immunoglobulin κ light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu. Immunity. 2003;19:203-11. (FC)
6. Heltemes-Harris L, Liu X, Manser T. Progressive surface B cell antigen receptor down-regulation accompanies efficient development of antinuclear antigen B cells to mature, follicular phenotype. J Immunol. 2004;172:823-33. (FC)
7. Wang Y, Carter RH. CD19 regulates B cell maturation, proliferation, and positive selection in the FDC zone of murine splenic germinal centers. Immunity. 2005;22:749-61. (FC)
8. Vettermann C, Lutz J, Selg M, Bösl M, Jäck H. Genomic suppression of murine B29/Ig-β promoter-driven transgenes. Eur J Immunol. 2006;36:3324-33. (FC)
9. Lindsley RC, Thomas M, Srivastava B, Allman D. Generation of peripheral B cells occurs via two spatially and temporally distinct pathways. Blood. 2007;109:2521-8. (FC)
10. Bai L, Chen Y, He Y, Dai X, Lin X, Wen R, et al. Phospholipase Cγ2 contributes to light-chain gene activation and receptor editing. Mol Cell Biol. 2007;27:5957-67. (FC)
11. Liu X, Shen S, Manser T. Influence of B cell antigen receptor expression level on pathways of B cell tolerance induction. J Immunol. 2009;182:398-407. (FC)
12. Gross AJ, Lyandres JR, Panigrahi AK, Prak ET, DeFranco AL. Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance. J Immunol. 2009;182:5382-92. (FC)
13. Stumhofer JS, Tait ED, Quinn WJ 3rd, Hosken N, Spudy B, Goenka R, et al. A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat Immunol. 2010;11:1119-26. (FC)
14. Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, et al. Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011;85:3367-74. (FC)
15. Knoll M, Yanagisawa Y, Simmons S, Engels N, Wienands J, Melchers F, et al. The non-Ig parts of the VpreB and λ5 proteins of the surrogate light chain play opposite roles in the surface representation of the precursor B cell receptor. J Immunol. 2012;188:6010-7. (FC)
16. Nandakumar KS, Collin M, Olsén A, Nimmerjahn F, Blom AM, Ravetch JV, et al. Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis. Eur J Immunol. 2007;37:2973-82. (IHC-FS)
17. Naumann I, Kappler R, von Schweinitz D, Debatin K, Fulda S. Bortezomib primes neuroblastoma cells for TRAIL-induced apoptosis by linking the death receptor to the mitochondrial pathway. Clin Cancer Res. 2011;17:3204-18. (WB-NR)
18. Unterkircher T, Cristofanon S, Vellanki SH, Nonnenmacher L, Karpel-Massler G, Wirtz CR, et al. Bortezomib primes glioblastoma, including glioblastoma stem cells, for TRAIL by increasing tBid stability and mitochondrial apoptosis. Clin Cancer Res. 2011;17:4019-30. (WB-NR)
19. Campbell KS, Cambier JC. B lymphocyte antigen receptors (mIg) are non-covalently associated with a disulfide linked, inducibly phosphorylated glycoprotein complex. EMBO J. 1990;9:441-8. (IP)
20. Vainio O, Dunon D, Aïssi F, Dangy J, McNagny KM, Imhof BA. HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors. J Cell Biol. 1996;135:1655-68. (Purification)
21. Tunheim G, Schjetne KW, Fredriksen AB, Sandlie I, Bogen B. Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes. J Leukoc Biol. 2005;77:303-10. (Purification)
22. Kisielow M, Kisielow J, Capoferri-Sollami G, Karjalainen K. Expression of lymphocyte activation gene 3 (LAG-3) on B cells is induced by T cells. Eur J Immunol. 2005;35:2081-8. (Costim)