Mouse Anti-Phosphotyrosine-HRP

Cat. No.
1400-05  
Size
1.0 mL  
Price (USD)
$230.00 
Clone PY20
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-HRP
Immunogen Phosphotyrosine conjugated to carrier proteins
Specificity Tyrosine-phosphorylated proteins
Alternalte Name(s) pTyr
Description Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate.
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1-4
FLISA – Quality tested
Western Blot – Reported in literature 1,5-7
Immunoprecipitation – Reported in literature 5
Immunohistochemistry-Paraffin Sections – Reported in literature 8
Immunohistochemsitry-Whole Mount – Reported in literature 9
Immunocytochemistry – Reported in literature 10
Flow Cytometry – Reported in literature 11
Microarray – Reported in literature 12,13
Purification – Reported in literature 1,14
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794751
  • ELISA plate was coated with BSA and BSA conjugated to phosphotyrosine (pTry-BSA). Phosphotyrosine was detected with serially diluted Mouse Anti-Phosphotyrosine-HRP (SB Cat. No. 1400-05).

ELISA plate was coated with BSA and BSA conjugated to phosphotyrosine (pTry-BSA). Phosphotyrosine was detected with serially diluted Mouse Anti-Phosphotyrosine-HRP (SB Cat. No. 1400-05).
 
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References
1. Glenney JR Jr, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988;109:277-85. (Immunogen, ELISA, WB, Purification)
2. Ruff-Jamison S, Campos-González R, Glenney JR Jr. Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features. J Biol Chem. 1991;266:6607-13. (ELISA)
3. Lietha D, Eck MJ. Crystal structures of the FAK kinase in complex with TAE226 and related bis-anilino pyrimidine inhibitors reveal a helical DFG conformation. PLoS One. 2008;3(11):e3800. (ELISA)
4. Antila H, Autio H, Turunen L, Harju K, Tammela P, Wennerberg K, et al. Utilization of in situ ELISA method for examining Trk receptor phosphorylation in cultured cells. J Neurosci Methods. 2014;222:142-6. (ELISA)
5. Vouri K, Ruoslahti E. Tyrosine phosphorylation of p130Cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix. J Biol Chem. 1995;270:22259-62. (WB, IP)
6. Elliott S, Egrie J, Browne J, Lorenzini T, Busse L, Rogers N, et al. Control of rHuEPO biological activity: the role of carbohydrate. Exp Hematol. 2004;32:1146-55. (WB)
7. Wang X, Cahill ME, Werner CT, Christoffel DJ, Golden SA, Xie Z, et al. Kalirin-7 mediates cocaine-induced AMPA receptor and spine plasticity, enabling incentive sensitization. J Neurosci. 2013;33:11012-22. (WB)
8. Haralambieva E, Jones M, Roncador GM, Cerroni L, Lamant L, Ott G, et al. Tyrosine phosphorylation in human lymphomas. Histochem J. 2002;34:545-52. (IHC-PS)
9. Gorczyca M, Augart C, Budnik V. Insulin-like receptor and insulin-like peptide are localized at neuromuscular junctions in Drosophila. J Neurosci. 1993;13:3692-704. (IHC-WM)
10. Takagi S, Daibata M, Last TJ, Humphreys RE, Parker DC, Sairenji T. Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells. J Exp Med. 1991;174:381-8. (ICC)
11. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000;39:250-9. (FC)
12. Sikkema AH, de Bont ES, Molema G, Dimberg A, Zwiers PJ, Diks SH, et al. Vascular endothelial growth factor receptor 2 (VEGFR-2) signalling activity in paediatric pilocytic astrocytoma is restricted to tumour endothelial cells. Neuropathol Appl Neurobiol. 2011;37:538-48. (Microarray)
13. Rosenberger AF, Hilhorst R, Coart E, García Barrado L, Naji F, Rozemuller AJ, et al. Protein kinase activity decreases with higher braak stages of alzheimer's disease pathology. J Alzheimers Dis. 2016;49:927-43. (Microarray)
14. Glenney JR Jr, Zokas L. Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton. J Cell Biol. 1989;108:2401-8. (Purification)