Hamster Anti-Mouse CD28-SPRD

Cat. No.
1615-13  
Size
0.1 mg  
Price (USD)
$245.00 
Clone PV-1
Isotype Hamster (Armenian) IgG2
Isotype Control Hamster IgG-SPRD
Immunogen C57BL/6N mouse T cell lymphoma EL4 cell line
Specificity Mouse CD28
Alternalte Name(s) Tp44, T44
Description CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Supplied in PBS/NaN3 and a stabilizing agent
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,6
Immunoprecipitation – Reported in literature 1,2
Costimulation – Reported in literature 1,3,4
Stimulation – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
  • C57BL/6N T lymphocyte cell line EL-4 was stained with Hamster Anti-Mouse CD28-PE (SB Cat. No. 1615-09L).

C57BL/6N T lymphocyte cell line EL-4 was stained with Hamster Anti-Mouse CD28-PE (SB Cat. No. 1615-09L).
 
References
1. Abe R, Vandenberghe P, Craighead N, Smoot DS, Lee KP, June CH. Distinct signal transduction in mouse CD4+ and CD8+ splenic T cells after CD28 receptor ligation. J Immuno. 1995;154:985-97. (Immunogen, FC, IP, Costim)
2. Dodson LF, Boomer JS, Deppong CM, Shah DD, Sim J, Bricker TL, et al. Targeted knock-in mice expressing mutations of CD28 reveal an essential pathway for costimulation. Mol Cell Biol. 2009;29:3710-21. (IP)
3. Kanzaki M, Wada J, Sugiyama K, Nakatsuka A, Teshigawara S, Murakami K, et al. Galectin-9 and T cell immunoglobulin mucin-3 pathway is a therapeutic target for type 1 diabetes. Endocrinology. 2012;153:612-20. (Costim)
4. Tomimori Y, Tanaka Y, Goto M, Fukuda Y. Repeated topical challenge with chemical antigen elicits sustained dermatitis in NC/Nga mice in specific-pathogen-free condition. J Invest Dermatol. 2005;124:119-24. (Costim)
5. Zhang T, Fresnay S, Welty E, Sangrampurkar N, Rybak E, Zhou H, et al. Selective CD28 blockade attenuates acute and chronic rejection of murine cardiac allografts in a CTLA-4-dependent manner. Am J Transplant. 2011;11:1599-1609. (Stim)
6. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)
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Spectral Red® is a PE/CY5 tandem conjugate
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