Rat Anti-Mouse CD45R-PE

Cat. No.
1665-09L  
Size
0.2 mg  
Price (USD)
$138.00 
Clone RA3-6B2
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-PE
Immunogen Abelson murine leukemia virus-induced pre-B cell lymphoma
Specificity Mouse/Human/Feline CD45R
Alternalte Name(s) B220, Ly-5, Lyt-4, Ptprc, Receptor-type tyrosine-protein phosphatase C
Description CD45R, also known as B220, a member of the protein tyrosine phosphate family and a major cell surface glycoprotein, represents a restricted form of the CD45 family which primarily recognizes only cells of B-lineage from pro-B cell through mature B lymphocytes and prior to the availability of anti-CD19 monoclonal antibodies was commonly used as a pan B-cell marker. It also reacts with certain activated T cells as well as non-MHC-restricted lytically active lymphokine-activated killer (LAK) cells. In vivo administration of RA3-6B2 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3,7-12
Immunohistochemistry-Frozen Sections – Reported in literature 3,5,6
Immunohistochemistry-Paraffin Sections – Reported in literature 3,4
Immunoprecipitation – Reported in literature 1,2
Western Blot – Reported in literature 2
Blocking – Reported in literature 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-PE (SB Cat. No. 1665-09L) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-PE (SB Cat. No. 1665-09L) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02).
 
References
1. Coffman RL. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 1982;69:5-23. (Immunogen, FC, IP, Block, Human Reactivity)
2. Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004;172:4298-307. (IP, WB, Human Reactivity)
3. Monteith CE, Chelack BJ, Davis WC, Haines DM. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 1996;60:193-8. (IHC-FS, IHC-PS, FC, Feline Reactivity)
4. Fütterer A, Campanero MR, Leonardo E, Criado LM, Flores JM, Hernández JM, et al. Dido gene expression alterations are implicated in the induction of hematological myeloid neoplasms. J Clin Invest. 2005;115:2351-62. (IHC-PS)
5. Konno A, Takada K, Saegusa J, Takiguchi M. Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjörgren's syndrome. Autoimmunity. 2003;36:247-54. (IHC-FS)
6. Haub S, Ritze Y, Bergheim I, Pabst O, Gershon MD, Bischoff SC. Enhancement of intestinal inflammation in mice lacking interleukin 10 by deletion of the serotonin reuptake transporter. Neurogastroenterol Motil. 2010;22:826-34. (IHC-FS)
7. Nepal RM, Tong L, Kolaj B, Edelmann W, Martin A. Msh2-dependent DNA repair mitigates a unique susceptibility of B cell progenitors to c-Myc-induced lymphomas. Proc Natl Acad Sci USA. 2009;106:18698-703. (FC)
8. Birjandi SZ, Ippolito JA, Ramadorai AK, Witte PL. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 2011;186:3441-51. (FC)
9. King RG, Herrin BR, Justement LB. Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response. J Immunol. 2011;187:3565-77. (FC)
10. Hinze A, Stolzing A. Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cells. J Inflamm. 2012;9:12. (FC)
11. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
12. Alkhatib A, Werner M, Hug E, Herzog S, Eschbach C, Faraidun H, et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med. 2012;209:395-406. (FC)
13. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)
14. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)