Mouse Anti-Rat IgG1-BIOT

Cat. No.
3060-08  
Size
0.5 mg  
Price (USD)
$210.00 
Clone G17E7
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-BIOT
Immunogen Rat IgG1 hybridoma
Specificity Rat IgG1
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-5
ELISA – Quality tested 6,7
FLISA – Quality tested
Immunohistochemistry-Frozen Sections 8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795857
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD22-UNLB (SB Cat. No. 1580-01) followed by Mouse Anti-Rat IgG1-BIOT (SB Cat. No. 3060-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
  • ELISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG1-BIOT (SB Cat. No. 3060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD22-UNLB (SB Cat. No. 1580-01) followed by Mouse Anti-Rat IgG1-BIOT (SB Cat. No. 3060-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
 

ELISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG1-BIOT (SB Cat. No. 3060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Hondowicz BD, Alexander ST, Quinn WJ 3rd, Pagán AJ, Metzgar MH, Cancro MP, et al. The role of BLyS/BLyS receptors in anti-chromatin B cell regulation. Int Immunol. 2007;19:465-75. (FC)
2. Treml LS, Carlesso G, Hoek KL, Stadanlick JE, Kambayashi T, Bram RJ, et al. TLR stimulation modifies BLyS receptor expression in follicular and marginal zone B cells. J Immunol. 2007;178:7531-9. (FC)
3. Crowley JE, Stadanlick JE, Cambier JC, Cancro MP. FcγRIIB signals inhibit BLyS signaling and BCR-mediated BLyS receptor up-regulation. Blood. 2009;113:1464-73. (FC)
4. Fink DR, Holm D, Schlosser A, Nielsen O, Latta M, Lozano F, et al. Elevated numbers of SCART1+ γδ T cells in skin inflammation and inflammatory bowel disease. Mol Immunol. 2010;47:1710-8. (FC)
5. Sang A, Niu H, Cullen J, Choi SC, Zheng YY, Wang H, et al. Activation of rheumatoid factor-specific B cells is antigen dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model. J Immunol. 2014;193:1609-21. (FC)
6. Madsen JL, Kroghsbo S, Madsen CB, Pozdnyakova I, Barkholt V, Bøgh KL. The impact of structural integrity and route of administration on the antibody specificity against three cow's milk allergens - a study in Brown Norway rats. Clin Transl Allergy. 2014;4:25. (ELISA)
7. Kroghsbo S, Andersen NB, Rasmussen TF, Jacobsen S, Madsen CB. Acid hydrolysis of wheat gluten induces formation of new epitopes but does not enhance sensitizing capacity by the oral route: a study in "gluten free" Brown Norway rats. PLoS One. 2014;9(9):e107137. (ELISA)
8. SouthernBiotech published data (IHC-FS)