Mouse Anti-Rat IgG2a-Alexa Fluor® 647

Cat. No.
3065-31  
Size
0.1 mg  
Price (USD)
$250.00 
Clone 2A8F4
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-Alexa Fluor® 647
Immunogen Rat IgG2a hybridoma
Specificity Rat IgG2a
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-5
ELISA – Quality tested 6
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 7
ELISpot – Reported in literature 8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795878
  • FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG2a-AF647 (SB Cat. No. 3065-31).
  • Con-A stimulated BALB/c mouse splenocytes were stained with Rat Anti-Mouse Ly-6A/E-UNLB (SB Cat. No. 1755-01) followed by Mouse Anti-Rat IgG2a-AF647 (SB Cat. No. 3065-31).

FLISA plate was coated with purified rat IgG1, IgG2a, IgG2b, IgG2c, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rat IgG2a-AF647 (SB Cat. No. 3065-31).
 

Con-A stimulated BALB/c mouse splenocytes were stained with Rat Anti-Mouse Ly-6A/E-UNLB (SB Cat. No. 1755-01) followed by Mouse Anti-Rat IgG2a-AF647 (SB Cat. No. 3065-31).
 
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References
1. Kim DD, Miwa T, Song W. Retrovirus-mediated over-expression of decay-accelerating factor rescues Crry-deficient erythrocytes from acute alternative pathway complement attack. J Immunol. 2006;117:5558-66. (FC)
2. Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, Song WC. Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg. Blood. 2008;112:1109-19. (FC)
3. Troeger C, Perahud I, Moser S, Holzgreve W. Transplacental traffic after in utero mesenchymal stem cell transplantation. Stem Cells Dev. 2010;19:1385-92. (FC)
4. Herbst T, Esser J, Prati M, Kulagin M, Stettler R, Zaiss MM, et al. Antibodies and IL-3 support helminth-induced basophil expansion. Proc Natl Acad Sci USA. 2012;109:14954-9. (FC)
5. Naik E, Webster JD, DeVoss J, Liu J, Suriben R, Dixit VM. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp Med. 2014;211:1947-55. (FC)
6. Du F, Lü L, Fu Q, Dai M, Teng J, Fan W, et al. T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis. Arthritis Res Ther. 2008;10:R136. (ELISA)
7. Birjandi SZ, Ippolito JA, Ramadorai AK, Witte PL. Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 2011;186:3441-51. (IHC-FS)
8. Sharkar MT, Jou M, Hossain MB, Lönnerdal B, Stephensen CB, Raqib R. Prenatal zinc supplementation of zinc-adequate rats adversely affects immunity in offspring. J Nutr. 2011;141:1559-64. (ELISPOT)
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