Mouse Anti-Chicken CD28-UNLB

Cat. No.
8260-01  
Size
0.5 mg  
Price (USD)
$240.00 
Clone AV7
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Chicken peripheral blood lymphocytes
Specificity Chicken/Turkey CD28
Description Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on antigen presenting cells. Peripheral γδ+ T cells are CD28 negative. Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A, or monoclonal antibodies against the avian T cell receptor (TCR). Both Vβ1- and Vβ2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A but inhibited by herbimycin A suggesting that CD28 employs a signaling pathway at least partly distinct from that triggered by TCR/CD3. The monoclonal antibody AV7 also reacts with turkey CD28 as demonstrated by flow cytometry.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,5-7
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunocytochemistry – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Costimulation – Reported in literature 4
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD28-PE (SB Cat. No. 8260-09) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD28-PE (SB Cat. No. 8260-09) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).
 
References
1. Young JR, Davison TF, Tregaskes CA, Rennie MC, Vainio O. Monomeric homologue of mammalian CD28 is expressed on chicken T cells. J Immunol. 1994;152:3848-51. (Immunogen, FC, IP)
2. Powell FL, Rothwell L, Clarkson MJ, Kaiser P. The turkey, compared to the chicken, fails to mount an effective early immune response to Histomonas meleagridis in the gut. Parasite Immunol. 2009;31:312-27. (IHC-FS, Turkey Reactivity)
3. Powell F, Lawson M, Rothwell L, Kaiser P. Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8α and CD28. Dev Comp Immunol. 2009;33:540-6. (ICC, Turkey Reactivity)
4. Peters MA, Browning GF, Washington EA, Crabb BS, Kaiser P. Embryonic age influences the capacity for cytokine induction in chicken thymocytes. Immunology. 2003;110:358-67. (Costim)
5. Meyerhoff RR, Ali RA, Liu K, Huang G, Koci MD. Comprehensive analysis of commercially available mouse antichicken monoclonal antibodies for cross-reactivity with peripheral blood leukocytes from commercial turkeys. Poult Sci. 2012;91:383-92. (FC, Turkey Reactivity)
6. Fernandes Filho T, Fávaro C Jr, Ingberman M, Beirão BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)
7. Machado PC Jr, Beirão BC, Filho TF, Lourenço MC, Joineau ML, Santin E, et al. Use of blends of organic acids and oregano extracts in feed and water of broiler chickens to control Salmonella Enteritidis persistence in the crop and ceca of experimentally infected birds. J Appl Poult Res. 2014;23:671-82. (FC)