Mouse F(ab')2 Anti-Human CD20-PE

Cat. No.
9352-09  
Size
100 tests  
Price (USD)
$305.00 
Clone B-Ly1
Isotype Mouse F(ab')2 IgG1κ
Isotype Control Mouse F(ab')2 IgG1-PE
Specificity Human CD20
Alternalte Name(s) Leu-16, B1, Bp35, MS4A1, S7
Description CD20 is a 33-37 kDa unglycosylated phosphoprotein. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. It is expressed on all stages of B cell differentiation except plasma cells. The CD20 molecule is involved in regulation of B cell differentiation presumably via its reported function as a Ca++ channel subunit.
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 5,6,8,9
Immunohistochemistry-Frozen Sections – Reported in literature 1,2
Immunohistochemistry-Paraffin Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 4
Blocking – Reported in literature 5
Separation – Reported in literature 6
Cytoxicity – Reported in literature 7
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796774
Gene ID
931 (Human)
Gene Symbol
MS4A1 (Human)
Gene Aliases B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Uniprot ID
P11836 (Human)
Uniprot Name
  • Human peripheral blood lymphocytes were stained with Mouse F(ab’)2 Anti-Human CD20-FITC (SB Cat. No. 9352-02) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09).

Human peripheral blood lymphocytes were stained with Mouse F(ab’)2 Anti-Human CD20-FITC (SB Cat. No. 9352-02) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09).
 
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References
1. Kang YM, Zhang X, Wagner UG, Yang H, Beckenbaugh RD, Kurtin PJ, et al. CD8 T cells are required for the formation of ectopic germinal centers in rheumatoid synovitis. J Exp Med. 2002;195:1325-36. (IHC-FS)
2. Yoon S, Zhang X, Lee IY, Spencer N, Vo P, Choi YS. CD9 is a novel marker for plasma cell precursors in human germinal centers. Biochem Biophys Res Commun. 2013;431:41-6. (IHC-FS)
3. Yang M, Xu Z, Zhuang Z. Macrophages affect immune inflammation and proliferation in benign prostatic hyperplasia via androgen receptor and CD40/CD40L signaling pathway. Tissue Cell. 2020;64:101343. (IHC-PS)
4. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD20. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al, editors. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989. p. 46-48. (IP)
5. Tedder TF, Penta A. Structure of the CD20 antigen and gene of human and mouse B-cells: use of transfected cell lines to examine the workshop panel of antibodies. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al, editors. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989. p. 48-50. (FC, Block)
6. Pawelec G, Müller R, Rehbein A, Hähnel K, Ziegler BL. Extrathymic T cell differentiation in vitro from human CD34+ stem cells. J Leukoc Biol. 1998;64:733-9. (FC, Sep)
7. Mössner E, Brünker P, Moser S, Püntener U, Schmidt C, Herter S, et al. Increasing the efficacy of CD20 antibody therapy through the engineering of a new type II anti-CD20 antibody with enhanced direct and immune effector cell-mediated B-cell cytotoxicity. Blood. 2010;115:4393-402. (Cyto)
8. Odendahl M, Jacobi A, Hansen A, Feist E, Hiepe F, Burmester GR, et al. Disturbed peripheral B lymphocyte homeostasis in systemic lupus erythematosus. J Immunol. 2000;165:5970-9. (FC)
9. Odendahl M, Keitzer R, Wahn U, Hiepe F, Radbruch A, Dörner T, et al. Perturbations of peripheral B lymphocyte homoeostasis in children with systemic lupus erythematosus. Ann Rheum Dis. 2003;62:851-8. (FC)