Mouse Anti-Human CD44-LE/AF

Cat. No.
9400-14  
Size
0.5 mg  
Price (USD)
$375.00 
Clone F10-44-2
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-LE/AF
Immunogen Purified T cells from human lymph nodes
Specificity Human/Ma’s Night Monkey CD44
Alternalte Name(s) Pgp-1, H-CAM, ECMR-III, HUTCH-1
Description CD44, also known as phagocytic glycoprotein-1 (Pgp-1), exists as a large number of different isoforms resulting from alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type I transmembrane protein. Other variable isoforms containing glycosaminoglycans have molecular weights ranging from 110 to 250 kDa and are widely expressed on hematopoietic and non-hematopoietic cells. CD44 contributes to the adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix. It also plays a functional role in cell migration, lymphocyte homing, and adhesion during hematopoiesis and lymphocyte activation.
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling -20°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2,9-16
Immunohistochemistry-Frozen Sections – Reported in literature 2-4
Immunohistochemistry-Paraffin Sections – Reported in literature 5,6
Immunocytochemistry – Reported in literature 7,8
Immunoprecipitation – Reported in literature 10,12,17,18
Western Blot – Reported in literature 7,19,20
Adhesion – Reported in literature 20,23
Activation – Reported in literature 20-22
Block – Reported in literature 23
Cytotoxicity – Reported in literature 2
CMCD – Reported in literature 24
Depletion – Reported in literature 12
Purification – Reported in literature 9,11
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796784
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD44-APC (SB Cat. No. 9400-11).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD44-APC (SB Cat. No. 9400-11).
 
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References
1. Dalchau R, Kirkley J, Fabre JW. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980;10:737-44. (Immunogen)
2. Dalchau R, Kirkley J, Fabre JW. Monoclonal antibody to a human brain-granulocyte-T lymphocyte antigen probably homologous to the W 3/13 antigen of the rat. Eur J Immunol. 1980;10:745-9. (FC, IHC-FS, Cyto)
3. Daar AS, Fabre JW. Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas. Lancet. 1981;2:434-8. (IHC-FS)
4. Ellis PA, Hart DN, Colls BM, Nimmo JC, MacDonald JE, Angus HB. Hodgkin's cells express a novel pattern of adhesion molecules. Clin Exp Immunol. 1992;90:117-23. (IHC-FS)
5. Krajewski AS, Howie S, O'Grady J, Biddlestone LR, Bellamy CO. Immunohistochemical staining of paraffin sections of lymphoid and epithelial tissues with workshop adhesion structure subpanel 10 (CD44) mAb. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al, editors. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1995. p. 1740-2. (IHC-PS)
6. Anwar F, Wood BL. CD44H and CD44V6 expression in different subtypes of Hodgkin lymphoma. Mod Pathol. 2000;13:1121-7. (IHC-PS)
7. Fukuda Y, Kurihara N, Imoto I, Yasui K, Yoshida M, Yanagihara K, et al. CD44 is a potential target of amplification within the 11p13 amplicon detected in gastric cancer cell lines. Genes Chromosomes Cancer. 2000;29:315-24. (ICC, WB)
8. Mathieu S, Gerolami R, Luis J, Carmona S, Kol O, Crescence L, et al. Introducing α(1,2)-linked fucose into hepatocarcinoma cells inhibits vasculogenesis and tumor growth. Int J Cancer. 2007;121:1680-9. (ICC)
9. Goodfellow PN, Banting G, Wiles MV, Tunnacliffe A, Parkar M, Solomon E, et al. The gene, MIC4, which controls expression of the antigen defined by monoclonal antibody F10.44.2, is on human chromosome 11. Eur J Immunol. 1982;12:659-63. (FC, Purification)
10. Stamenkovic I, Aruffo A, Amiot M, Seed B. The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO J. 1991;10:343-8. (FC, IP)
11. Dalchau R, Flanagan BF, Allen AK, Daar AS, Fabre JW. Chemical composition and tissue distribution of the human CD44 glycoprotein. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al, editors. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989. p. 622-5. (FC, Purification)
12. Bartolazzi A, Jackson D, Bennett K, Aruffo A, Dickinson R, Shields J, et al. Regulation of growth and dissemination of a human lymphoma by CD44 splice variants. J Cell Sci. 1995;108:1723-33. (FC, IP, Depletion)
13. Daubenberger CA, Spirig R, Patarroyo ME, Pluschke G. Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research. Vet Immunol Immunopathol. 2007;119:14-20. (FC, Ma's Night Monkey Reactivity)
14. Hayashi S, Fujita K, Matsumoto S, Akita M, Satomi A. Isolation and identification of cancer stem cells from a side population of a human hepatoblastoma cell line, HuH-6 clone-5. Pediatr Surg Int. 2011;27:9-16. (FC)
15. Szöke K, Beckstrøm KJ, Brinchmann JE. Human adipose tissue as a source of cells with angiogenic potential. Cell Transplant. 2012;21:235-50. (FC)
16. Pullisaar H, Verket A, Szoke K, Tiainen H, Haugen HJ, Brinchmann JE, et al. Alginate hydrogel enriched with enamel matrix derivative to target osteogenic cell differentiation in TiO2 scaffolds. J Tissue Eng. 2015;6:2041731415575870. (FC)
17. Letarte M. Human p85 glycoprotein bears three distinct epitopes defined by several monoclonal antibodies. Mol Immunol. 1986;6:639-44. (IP)
18. Mackay CR, Maddox JF, Wijffels GL, Mackay IR, Walker ID. Characterization of a 95,000 molecule on sheep leucocytes homologous to murine Pgp-1 and human CD44. Immunology. 1988;65:93-9. (IP)
19. Štefanová I, Hilgert I, Bažil V, Angelisová P, Hořejší V. Reactivity of the non-lineage/NK panel antibodies with purified antigens CD45, CD43, CD44, and CD18, and three 'novel' antigens MEM-43, MEM-53, and MEM-102. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, et al, editors. Leukocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989. p. 678-80. (WB)
20. Takahashi K, Eto H, Tanabe KK. Involvement of CD44 in matrix metalloproteinase-2 regulation in human melanoma cells. Int J Cancer. 1999;80:387-95. (WB, Activ, Adhesion)
21. Denning SM, Telen MJ, Hale LP, Liao HX, Haynes BF. CD44 and CD44R cluster report. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al, editors. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1995. p. 1713-9. (Activ)
22. Murray D, Morrin M, McDonnell S. Increased invasion and expression of MMP-9 in human colorectal cell lines by a CD44-dependent mechanism. Anticancer Res. 2004;24:489-94. (Activ)
23. Ruile P, Rank G, Rieber EP. mAb inhibiting the adherence of germinal centre B lymphocytes to follicular dendritic. In: Schlossman SF, Boumsell L, Gilks W, Harlan JM, Kishimoto T, Morimoto C, et al, editors. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1995. p. 1576-7. (Adhesion, Block)
24. Chapman JR, Taylor CJ, Carter NP, Morris PJ. Comparison of non-lineage antibodies with cytotoxic autoreactive antibodies in haemodialysis patients. In: McMichael AJ, Beverley PC, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al, editors. Leukocyte Typing III: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1987. p. 826-8. (CMCD)