Mouse Anti-Human CD62E/CD62P-SPRD

Cat. No.
9500-13  
Size
100 tests  
Price (USD)
$250.00 
Clone 1.2B6
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-SPRD
Immunogen TNF stimulated human endothelial cells
Specificity Human CD62E & CD62P/Porcine CD62E
Alternalte Name(s) E-selectin, P-selectin, ELAM-1, LECAM-2, PADGEM, GMP-140, LECAM-3
Description CD62E represents the 97 kDa type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues.

CD62P is a 140 kDa membrane glycoprotein formerly known as platelet activation-dependent granule membrane protein (PADGEM) or GMP-140. It is expressed on platelets, megakaryocytes, and epithelium. It is thought to mediate the adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses and of activated platelets to neutrophils and monocytes in hemostasis.

The monoclonal antibody 1.2B6 recognizes a common epitope shared by CD62E and CD62P.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2,5,10-17
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunocytochemistry – Reported in literature 5
Immunoprecipitation – Reported in literature 1
Western Blot-Non-Reducing – Reported in literature 2,3
ELISA – Reported in literature 1,6-8
Blocking – Reported in literature 9,10
Adhesion – Reported in literature 9,10
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796820
Gene ID
6401 (Human)
6403 (Human)
397508 (Porcine)
Gene Symbol
SELE (Human)
SELP (Human)
SELE (Porcine)
Gene Aliases CD62E; ELAM; ELAM1; ESEL; LECAM2; CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
Uniprot ID
P16581 (Human)
P16109 (Human)
P98110 (Porcine)
Uniprot Name
  • PMA and ionomycin stimulated human endothelial cell line HUV-EC-C was stained with Mouse Anti-Human CD62E/CD62P-SPRD (SB Cat. No. 9500-13).

PMA and ionomycin stimulated human endothelial cell line HUV-EC-C was stained with Mouse Anti-Human CD62E/CD62P-SPRD (SB Cat. No. 9500-13).
 
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References
1. Wellicome SM, Thornhill MH, Pitzalis C, Thomas DS, Lanchbury JS, Panayi GS, et al. A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. J Immunol. 1990;144:2558-65. (Immunogen, FC, IP, ELISA)
2. Goda K, Tanaka T, Monden M, Miyasaka M. Characterization of an apparently conserved epitope in E- and P-selectin identified by dual-specific monoclonal antibodies. Eur J Immunol. 1999;29:1551-60. (FC, WB)
3. DeLisser HM, Christofidou-Solomidou M, Sun J, Nakada MT, Sullivan KE. Loss of endothelial surface expression of E-selectin in a patient with recurrent infections. Blood. 1999;94:884-94. (WB)
4. Ku C, Zerboni L, Ito H, Graham BS, Wallace M, Arvin AM. Varicella-zoster virus transfer to skin by T cells and modulation of viral replication by epidermal cell interferon-α. J Exp Med. 2004;200:917-25. (IHC-FS)
5. Akinci MA, Turner H, Taveras M, Barash A, Wang Z, Reinach P, et al. Molecular profiling of conjunctival epithelial side-population stem cells: atypical cell surface markers and sources of a slow-cycling phenotype. Invest Ophthalmol Vis Sci. 2009;50:4162-72. (ICC, FC)
6. Kalogeris TJ, Kevil CG, Laroux FS, Coe LL, Phifer TJ, Alexander JS. Differential monocyte adhesion and adhesion molecule expression in venous and arterial endothelial cells. Am J Physiol. 1999;276:L9-19. (ELISA)
7. Chen K, Chang BH, Younan P, Shlykov SG, Sanborn BM, Chan L. Increased intracellular calcium transients by calmodulin antagonists differentially modulate tumor necrosis factor-α-induced E-selectin and ICAM-1 expression. Atherosclerosis. 2002;165:5-13. (ELISA)
8. Kikuchi H, Isshi K, Hirohata S. Inhibitory effects of bucillamine on the expression of vascular cell adhesion molecule-1 in human umbilical vein endothelial cells. Int Immunopharmacol. 2004;4:119-26. (ELISA)
9. Tan PH, Chan C, Xue SA, Dong R, Ananthesayanan B, Manunta M, et al. Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells. Atherosclerosis. 2004;173:171-83. (Block, Adhesion)
10. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC, Block, Adhesion)
11. Breninschek RI, Oppenheimer-Marks N, Lipsky PE. Activated T cells acquire endothelial cell surface determinants during transendothelial migration. J Immunol. 1999;162:1677-84. (FC)
12. Roy V, Verfaillie CM. Expression and function of cell adhesion molecules on fetal liver, cord blood and bone marrow hematopoietic progenitors: implications for anatomical localization and developmental stage specific regulation of hematopoiesis. Exp Hematol. 1999;27:302-12. (FC)
13. Nohé B, Dieterich H, Eichner M, Unertl K. Certain batches of albumin solutions influence the expression of endothelial cell adhesion molecules. Intensive Care Med. 1999;25:1381-5. (FC)
14. Yamamoto H, Sedgwick JB, Vrtis RF, Busse WW. The effect of transendothelial migration on eosinophil function. Am J Respir Cell Mol Biol. 2000;23:379-88. (FC)
15. Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, et al. E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Int J Cancer. 2008;123:1741-50. (FC)
16. Sæthre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, et al. Cytokine secretion depends on Galα(1,3)Gal expression in a pig-to-human whole blood model. J Immunol. 2008;180:6346-53. (FC)
17. Cockerill GW, Huehns TY, Weerasinghe A, Stocker C, Lerch PG, Miller NE, et al. Elevation of plasma high-density lipoprotein concentration reduces interleukin-1-induced expression of E-selectin in an in vivo model of acute inflammation. Circulation. 2001;103:108-12. (FC, Porcine Reactivity)
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