Mouse Anti-Human CD57-Pacific Blue™

Cat. No.
9665-26  
Size
100 tests  
Price (USD)
$225.00 
Clone NK-1
Isotype Mouse IgMκ
Immunogen Membrane extract of the human lymphoblastoid cell line HSB-2
Specificity Human CD57
Alternalte Name(s) HNK-1, Leu-7, B3GAT1
Description CD57 represents a 110 kDa oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitan sulfate proteoglycans. It is expressed on 7-35% of normal peripheral blood lymphocytes, including subsets of NK cells and CD8+ T lymphocytes. The carbohydrate is expressed on various cell adhesion molecules in the nervous system and is suggested to play a role in cell–cell and cell–substrate interactions.
Format/Conjugate PACBLU (Pacific Blue™)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-4
Immunohistochemistry-Frozen Sections – Reported in literature 5
Immunohistochemistry-Paraffin Sections – Reported in literature 6-8
Immunocytochemistry – Reported in literature 2,3
Immunoprecipitation – Reported in literature 8
Western Blot – Reported in literature 2,3,8,9
Purification – Reported in literature 9
Blocking – Reported in literature 2,8
Adhesion – Reported in literature 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2797058
Gene ID
27087 (Human)
Gene Symbol
B3GAT1 (Human)
Gene Aliases CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
Uniprot ID
Q9P2W7 (Human)
Uniprot Name
  • Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-PACBLU (SB Cat. No. 9665-26) and Mouse Anti-Human CD3-PE (SB Cat. No. 9515-09).

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-PACBLU (SB Cat. No. 9665-26) and Mouse Anti-Human CD3-PE (SB Cat. No. 9515-09).
 
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References
1. Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol. 1981;127:1024-9. (Immunogen, FC)
2. Miura R, Ethell IM, Yamaguchi Y. Carbohydrate-protein interactions between HNK-1-reactive sulfoglucuronyl glycolipids and the proteoglycan lectin domain mediate neuronal cell adhesion and neurite outgrowth. J Neurochem. 2001;76:413-24. (FC, ICC, WB, Block, Adhesion)
3. Yanagisawa M, Taga T, Nakamura K, Ariga T, Yu RK. Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells. J Neurochem. 2005;95:1311-20. (FC, ICC, WB)
4. Bentebibel S, Schmitt N, Banchereau J, Ueno H. Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers. Proc Natl Acad Sci USA. 2011;108:E488-97. (FC)
5. Liu AY, Roudier MP, True LD. Heterogeneity in primary and metastatic prostate cancer as defined by cell surface CD profile. Am J Pathol. 2004;165:1543-56. (IHC-FS)
6. Jensen HK, Donskov F, Marcussen N, Nordsmark M, Lundbeck F, von der Maase H. Presence of intratumoral neutrophils is an independent prognostic factor in localized renal cell carcinoma. J Clin Oncol. 2009;27:4709-17. (IHC-PS)
7. Zou Y, Chen T, Han M, Wang H, Yan W, Song G, et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol. 2010;184:466-75. (IHC-PS)
8. Suzuki-Anekoji M, Suzuki M, Kobayashi T, Sato Y, Nakayama J, Suzuki A, et al. HNK-1 glycan functions as a tumor suppressor for astrocytic tumor. J Biol Chem. 2011;286:32824-33. (IHC-PS, IP, WB, Block)
9. Yoshihara Y, Oka S, Watanabe Y, Mori K. Developmentally and spatially regulated expression of HNK-1 carbohydrate antigen on a novel phosphatidylinositol-anchored glycoprotein in rat brain. J Cell Biol. 1991;115:731-44. (WB, Purification)
Pacific Blue™ is a trademark of Life Technologies Corporation