Human Type IV Collagen-Solution

Cat. No.
1250-01S  
Size
0.5 mg  
Price (USD)
$305.00 
Description Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type IV collagen is primarily expressed in the basal lamina and therefore constitutes a portion of the basement membrane. Type IV collagen mutations are associated with Alport syndrome and the α3(IV) chain is thought to be the antigen implicated in Goodpasture's syndrome. Type IV collagen is comprised of six isomeric chains designated α1(IV) to α6(IV).
Source Placental villi
Purity > 90% by SDS-PAGE
Purification Controlled and limited pepsin digestion followed by selective salt precipitation
Alternalte Name(s) COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, COL4A6
Buffer Formulation 500 mM acetic acid
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested 2-4
SDS-PAGE – Quality tested 1
Coating Material for –
Adhesion Studies – Reported in literature 5,6
Blood Disorder Studies – Reported in literature 2-4
Differentiation Studies – Reported in literature 7
ECM Interaction Studies – Reported in literature 8,9
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with serially diluted Human Type IV Collagen (SB Cat. No. 1250-01S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with serially diluted Human Type IV Collagen (SB Cat. No. 1250-01S). Purified collagen was detected with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08), Goat Anti-Type II Collagen-BIOT (SB Cat. No. 1320-08), Goat Anti-Type III Collagen-BIOT (SB Cat. No. 1330-08), Goat Anti-Type IV Collagen-BIOT (SB Cat. No. 1340-08), Goat Anti-Type V Collagen-BIOT (SB Cat. No. 1350-08), and Goat Anti-Type VI Collagen-BIOT (SB Cat. No. 1360-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Kim T, Moon JH, Savard CE, Kuver R, Lee SP. Effects of lipopolysaccharide on platelet-derived growth factor isoform and receptor expression in cultured rat common bile duct fibroblasts and cholangiocytes. J Gastroenterol Hepatol. 2009;24:1218-25. (SDS-PAGE)
2. Doruelo AL, Haberichter SL, Christopherson PA, Boggio LN, Gupta S, Lentz SR, et al. Clinical and laboratory phenotype variability in type 2M von Willebrand disease. J Thromb Haemost. 2017;15:1559-66. (ELISA, Coating, Blood Disorder Studies)
3. Garcia J, Flood VH, Haberichter SL, Fahs SA, Mattson JG, Geurts AM, et al. A rat model of severe VWD by elimination of the VWF gene using CRISPR/Cas9. Res Pract Thromb Haemost. 2019;4:64-71. (ELISA, Coating, Blood Disorder Studies)
4. Chen W, Voos KM, Josephson CD, Li R. Short-acting anti-VWF (von Willebrand factor) aptamer improves the recovery, survival, and hemostatic functions of refrigerated platelets. Arterioscler Thromb Vasc Biol. 2019;39:2028-37. (ELISA, Coating, Blood Disorder Studies)
5. Midwood KS, Salter DM. NG2/HMPG modulation of human articular chondrocyte adhesion to type VI collagen is lost in osteoarthritis. J Pathol. 2001;195:631-5. (Coating, Adhesion Studies)
6. Imakawa K, Imai M, Sakai A, Suzuki M, Nagaoka K, Sakai S, et al. Regulation of conceptus adhesion by endometrial CXC chemokines during the implantation period in sheep. Mol Reprod Dev. 2006;73:850-8. (Coating, Adhesion Studies)
7. Hohn H, Denker H. The role of cell shape for differentiation of choriocarcinoma cells on extracellular matrix. Exp Cell Res. 1994;215:40-50. (Coating, Differentiation Studies)
8. Bidanset DJ, Guidry C, Rosenberg LC, Choi HU, Timpl R, Hook M. Binding of the proteoglycan decorin to collagen type VI. J Biol Chem. 1992;267:5250-6. (Coating, ECM Interaction Studies)
9. Liu Z, Han X, Chen R, Zhang K, Li Y, Fruge S, et al. Microfluidic mapping of cancer cell-protein binding interaction. ACS Appl Mater Interfaces. 2017;9:22143-8. (Coating, ECM Interaction Studies)