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SBA Clonotyping System-C57BL/6-HRP

Cat. No.
5300-05B

Size
1 kit

Price (USD)
$420.00

Qty	Add to Cart

Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Description	The SBA Clonotyping System-C57BL/6-HRP kit is designed for the isotyping of C57BL/6 mouse monoclonal antibodies. It contains 2.5 mg of capture antibody and 1.0 mL of HRP conjugated anti-mouse Ig, mouse IgA, mouse IgG₁, mouse IgG_2b, mouse IgG_2c, mouse IgG₃, mouse IgM, mouse κ, mouse λ, and ABTS substrate. The kit may also be utilized for quantitative studies of mouse immunoglobulins in samples such as serum when used in conjunction with the Mouse Immunoglobulin Panel (SB Cat. No. 5300-01B).
Kit Components	Goat Anti-Mouse Ig, Human ads-UNLB - 2.5 mg in 1.0 mL Goat Anti-Mouse Ig, Human ads-HRP - 1.0 mL Goat Anti-Mouse IgA-HRP - 1.0 mL Goat Anti-Mouse IgG₁, Human ads-HRP - 1.0 mL Goat Anti-Mouse IgG_2b, Human ads-HRP - 1.0 mL Goat Anti-Mouse IgG_2c, Human ads-HRP - 1.0 mL Goat Anti-Mouse IgG₃, Human ads-HRP - 1.0 mL Goat Anti-Mouse IgM, Human ads-HRP - 1.0 mL Goat Anti-Mouse Kappa-HRP - 1.0 mL Goat Anti-Mouse Lambda-HRP - 1.0 mL ABTS Substrate Powder - 100 mg
Buffer Formulation	UNLB antibody - Borate buffered saline, pH 8.2 HRP conjugates - 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	ELISA – Quality tested ^1-8
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796081

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References

1.	Chmielewski M, Hahn O, Rappl G, Nowak M, Schmidt-Wolf IH, Hombach AA, et al. T cells that target carcinoembryonic antigen eradicate orthotopic pancreatic carcinomas without inducing autoimmune colitis in mice. Gastroenterology. 2012;143:1095-107. (ELISA - Serum)
2.	Zhang X, Wu C, Song J, Götte M, Sorokin L. Syndecan-1, a cell surface proteoglycan, negatively regulates initial leukocyte recruitment to the brain across the choroid plexus in murine experimental autoimmune encephalomyelitis. J Immunol. 2013;191:4551-61. (ELISA - Serum)
3.	Patton DT, Plumb AW, Redpath SA, Osborne LC, Perona-Wright G, Abraham N. The development and survival but not function of follicular B cells is dependent on IL-7Rα Tyr449 signaling. PLoS One. 2014;9(2):e88771. (ELISA - Serum)
4.	Chen A, Geng Y, Ke H, Constant L, Yan Z, Pan Y, et al. Cutting edge: Dexamethasone potentiates the responses of both regulatory T cells and B-1 cells to antigen immunization in the ApoE^-/- mouse model of atherosclerosis. J Immunol. 2014;193:35-9. (ELISA - Serum)
5.	Hartweger H, Schweighoffer E, Davidson S, Peirce MJ, Wack A, Tybulewicz VL. Themis2 is not required for B cell development, activation, and antibody responses. J Immunol. 2014;193:700-7. (ELISA - Serum)
6.	Li X, Liang Y, LeBlanc M, Benner C, Zheng Y. Function of a Foxp3 cis-element in protecting regulatory T cell identity. Cell. 2014;158:734-48. (ELISA - Serum)
7.	Ray A, Yuan C, Miller NM, Mei H, Dittel BN. 2B4 is dispensable for T-dependent B cell immune responses, but its deficiency leads to enhanced T-independent responses due to an increase in peritoneal cavity B1b cells. PLoS One. 2015;10(8):e0137314. (ELISA - Serum)
8.	Kabat AM, Harrison OJ, Riffelmacher T, Moghaddam AE, Pearson CF, Laing A, et al. The autophagy gene Atg16l1 differentially regulates T_reg and T_H2 cells to control intestinal inflammation. Elife. 2016;5:e12444. (ELISA - Serum)

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