ELISA plate was coated with serially diluted Human IgA Lambda-UNLB (SB Cat. No. 0155L-01). Immunoglobulin was detected with Mouse Anti-Human IgG-BIOT (SB Cat. No. 9040-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA2-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.