Goat Anti-Type V Collagen-UNLB

Cat. No.
1350-01  
Size
0.2 mg  
Price (USD)
$395.00 
Specificity Reacts with conformational determinants on type V collagen
Source Pooled antisera from goats hyperimmunized with type V collagen
Cross Adsorption Collagen types I, II, III, IV, and VI
Purification Affinity chromatography on type V collagen covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.4 mg/mL
Volume 0.5 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 3-14
Immunohistochemistry-Frozen Sections 4,9,12,15-22
Immunocytohemistry 9,23,24
Electron Microscopy 25-27
Western Blot 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794740
Gene ID
1289 (Human)
1290 (Human)
50509 (Human)
Gene Symbol
COL5A1 (Human)
COL5A2 (Human)
COL5A3 (Human)
Gene Aliases EDSC; EDSCL1; EDSCL2
Uniprot ID
P20908 (Human)
P05997 (Human)
P25940 (Human)
Uniprot Name
  • Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF555 (SB Cat. No. 6420-32) and DAPI.
  • Frozen D-arginine treated Mdx mouse quadricep muscle section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by a secondary antibody.

    Image from Wehling-Henricks M, Jordan MC, Gotoh T, Grody WW, Roos KP, Tidball JG. Arginine metabolism by macrophages promotes cardiac and muscle fibrosis in mdx muscular dystrophy. PloS One. 2010;5(5):e10763. Figure 7
  • Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Mouse Anti-Goat IgG Fc-AF555 (SB Cat. No. 6158-32) and DAPI.
  • Paraffin embedded mouse inguinal mammary glands were stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by a secondary antibody and DAPI.

    Images from Huang G, Ge G, Izzi V, Greenspan DS. α3 chains of type V collagen regulate breast tumour growth via glypican-1. Nat Commun. 2017;8:14351. Figure 1(a)
  • Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6425-32) and DAPI.
  • Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.

Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF555 (SB Cat. No. 6420-32) and DAPI.
 

Frozen D-arginine treated Mdx mouse quadricep muscle section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by a secondary antibody.

Image from Wehling-Henricks M, Jordan MC, Gotoh T, Grody WW, Roos KP, Tidball JG. Arginine metabolism by macrophages promotes cardiac and muscle fibrosis in mdx muscular dystrophy. PloS One. 2010;5(5):e10763. Figure 7
 

Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Mouse Anti-Goat IgG Fc-AF555 (SB Cat. No. 6158-32) and DAPI.
 

Paraffin embedded mouse inguinal mammary glands were stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by a secondary antibody and DAPI.

Images from Huang G, Ge G, Izzi V, Greenspan DS. α3 chains of type V collagen regulate breast tumour growth via glypican-1. Nat Commun. 2017;8:14351. Figure 1(a)
 

Frozen newborn mouse cartilage section was stained with Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6425-32) and DAPI.
 

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type V Collagen-UNLB (SB Cat. No. 1350-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
 
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References
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