Goat Anti-Human IgD-BIOT

Cat. No.
2030-08  
Size
1.0 mg  
Price (USD)
$115.00 
Specificity Reacts with the heavy chain of human IgD
Source Pooled antisera from goats hyperimmunized with human IgD
Cross Adsorption Human IgG, IgM, and IgA; may react with IgD from other species
Purification Affinity chromatography on human IgD covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry 5-17
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1-4
Immunohistochemistry-Paraffin Sections 19
Separation 18
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795629
Gene ID
3495 (Human)
Gene Symbol
IGHD (Human)
Uniprot ID
P01880 (Human)
Uniprot Name
  • ELISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgD-BIOT (SB Cat. No. 2030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
  • Frozen human endocervical section was stained with Goat Anti-Human IgD-BIOT (SB Cat. No. 2030-08) followed by a secondary antibody and DAB.

    Image from Hirbod T, Kaldensjö T, Broliden K. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS One. 2011;6(9):e25551. Figure 4(d)
  • Human peripheral blood lymphocytes were stained with Goat Anti-Human IgD-BIOT (SB Cat. 2030-08) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

ELISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgD-BIOT (SB Cat. No. 2030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 

Frozen human endocervical section was stained with Goat Anti-Human IgD-BIOT (SB Cat. No. 2030-08) followed by a secondary antibody and DAB.

Image from Hirbod T, Kaldensjö T, Broliden K. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS One. 2011;6(9):e25551. Figure 4(d)
 

Human peripheral blood lymphocytes were stained with Goat Anti-Human IgD-BIOT (SB Cat. 2030-08) and Mouse Anti-Human CD19-PE (SB Cat. No. 9340-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
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19. SouthernBiotech unpublished data