Goat F(ab')2 Anti-Human IgA-FITC

Cat. No.
2052-02  
Size
0.5 mg  
Price (USD)
$95.00 
Specificity Reacts with the heavy chain of human IgA
Source Pepsin digest of Goat Anti-Human IgA (SB Cat. No. 2050)
Cross Adsorption Human IgG and IgM; may react with IgA from other species
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-FITC
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-4,6
FLISA
Flow Cytometry 9-16
Other referenced applications for relevant formats include -
ELISpot 5
Immunohistochemistry-Frozen Sections 7,8
Stimulation 17
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795710
  • FLISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human IgA-FITC (SB Cat. No. 2052-02).

FLISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat F(ab’)2 Anti-Human IgA-FITC (SB Cat. No. 2052-02).
 
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References
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2. Matousovic K, Novak J, Yanagihara T, Tomana M, Moldoveanu Z, Kulhavy R, et al. IgA-containing immune complexes in the urine of IgA nephropathy patients. Nephrol Dial Transplant. 2006;21:2478-84. (ELISA)
3. Huggins J, Pellegrin T, Felgar RE, Wei C, Brown M, Zheng B, et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Blood. 2007;109:1611-9. (ELISA)
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6. White CA, Pone EJ, Lam T, Tat C, Hayama KL, Li G, et al. Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses. J Immunol. 2014;193:5933-50. (ELISA)
7. Boumediene A, Oblet C, Oruc Z, Duchez S, Morelle W, Huynh A, et al. Gammopathy with IgA mesangial deposition provides a monoclonal model of IgA nephritogenicity and offers new insights into its molecular mechanisms. Nephrol Dial Transplant. 2011;26:3930-7. (IHC-FS)
8. Burgener A, Tjernlund A, Kaldensjo T, Abou M, McCorrister S, Westmacott GR, et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol. 2013;87:5141-50. (IHC-FS)
9. Grossetête B, Launay P, Lehuen A, Jungers P, Bach J, Monteiro RC. Down-regulation of Fcα receptors on blood cells of IgA nephropathy patients: evidence for a negative regulatory role of serum IgA. Kidney Int. 1998;53:1321-35. (FC)
10. Morton HC, van Zandbergen G, van Kooten C, Howard CJ, van de Winkel JG, Brandtzaeg P. Immunoglobulin-binding sites of human FcαRI (CD89) and bovine Fcγ2R are located in their membrane-distal extracellular domains. J Exp Med. 1999;189:1715-22. (FC)
11. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (FC)
12. van der Pol W, Vidarsson G, Vilé HA, van de Winkel JG, Rodriguez ME. Pneumococcal capsular polysaccharide-specific IgA triggers efficient neutrophil effector functions via FcαRI (CD89). J Infect Dis. 2000;182:1139-45. (FC)
13. Rodriguez ME, Hellwig SM, Hozbor DF, Leusen J, van der Pol W, van de Winkel JG. Fc receptor-mediated immunity against Bordetella pertussis. J Immunol. 2001;167:6545-51. (FC)
14. Honorio-Franca AC, Launay P, Carneiro-Sampaio MM, Monteiro RC. Colostral neutrophils express Fcα receptors (CD89) lacking γ chain association and mediate noninflammatory properties of secretory IgA. J Leukoc Biol. 2001;69:289-96. (FC)
15. Heystek HC, Moulon C, Woltman AM, Garonne P, van Kooten C. Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation. J Immunol. 2002;168:102-7. (FC)
16. Mota G, Manciulea M, Cosma E, Popescu I, Hirt M, Jensen-Jarolim E, et al. Human NK cells express Fc receptors for IgA which mediate signal transduction and target cell killing. Eur J Immunol. 2003;33:2197-205. (FC)
17. Toapanta FR, Bernal PJ, Sztein MB. Diverse phosphorylation patterns of B cell receptor-associated signaling in naïve and memory human B cells revealed by phosphoflow, a powerful technique to study signaling at the single cell level. Front Cell Infect Microbiol. 2012;2:128. (Stim)