Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-Alexa Fluor® 488

Cat. No.
6420-30  
Size
1.0 mg  
Price (USD)
$195.00 
Specificity Reacts with the heavy and light chains of goat IgG
Source Pooled antisera from donkeys hyperimmunized with goat IgG
Cross Adsorption Mouse and rat serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Affinity chromatography on goat IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 2,3
Immunohistochemistry-Frozen Sections 4
Immunohistochemistry-Paraffin Sections 11
Western Blot 5-9
Multiplex 10
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796338
  • Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF488 (SB Cat. No. 6420-30) and DAPI.

Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AF488 (SB Cat. No. 6420-30) and DAPI.
 
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References
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8. Gao H, Yallampalli U, Yallampalli C. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod. 2012;86:68. (WB)
9. Bouchez I, Pouteaux M, Canonge M, Genet M, Chardot T, Guillot A, et al. Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase. Biol Open. 2015;00:pii: bio.20148615. (WB)
10. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (Multiplex)
11. SouthernBiotech published data (IHC-PS)
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