Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP

Cat. No.
6425-05  
Size
1.0 mL  
Price (USD)
$125.00 
Specificity Reacts with the heavy and light chains of goat IgG
Source Pooled antisera from donkeys hyperimmunized with goat IgG
Cross Adsorption Human, mouse, rat, hamster, rabbit, chicken, horse, and guinea pig serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Affinity chromatography on goat IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1
Western Blot 1
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796346
  • Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
  • ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05).

Purified Human Type I Collagen (SB Cat. No. 1200-01S) was resolved by electrophoresis, transferred to PVDF membrane, probed with Goat Anti-Type I Collagen-UNLB (SB Cat. No. 1310-01), and visualized using Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05) secondary antibody and chemiluminescent detection.
 

ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6425-05).
 
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References
1. SouthernBiotech published data (IHC-FS, WB)