Donkey Anti-Rat IgG(H+L), Mouse SP ads-Alexa Fluor® 647

Cat. No.
6430-31  
Size
1.0 mg  
Price (USD)
$195.00 
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from donkeys hyperimmunized with rat IgG
Cross Adsorption Mouse serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Purification Affinity chromatography on rat IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Paraffin Sections 1
Immunocytochemistry 3
Western Blot 2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796358
  • NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-AF488 (SB Cat. No. 6430-30) and DAPI.

NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-AF488 (SB Cat. No. 6430-30) and DAPI.
 
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References
1. Basso K, Schneider C, Shen Q, Holmes AB, Setty M, Leslie C, et al. BCL6 positively regulates AID and germinal center gene expression via repression of miR-155. J Exp Med. 2012;209:2455-65. (IHC-PS)
2. SouthernBiotech unpublished data (WB)
3. SouthernBiotech published data (ICC)
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