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Antibody Purification Services | SouthernBiotech
 
The Antibody Company - SouthernBiotech
 
 
 

Antibody Purification Services

SouthernBiotech uses a variety of methods for purification of antibody from serum, ascites, and culture supernatants including
(NH4)2SO4 salt precipitation, ion exchange and size exclusion chromatography, Protein A and Protein G affinity chromatography, and custom affinity chromatography. The method employed is dictated by the species, the source material, and the level of immunoglobulin purity required. The amount of supernatant or serum required per column depends on the concentration and total milligrams of the solution.
Available Services for Antibody Conjugation Cat. No.
Affinity Purification SBCS-45
Antibodies of extremely high purity and specificity can be obtained using custom affinity chromatography. A minimum of 10 mg of pure antigen (or purified peptide) is needed to perform this procedure.
Ammonium Sulfate Precipitation SBCS-40
Ammonium sulfate is widely used in isolating both IgM and IgG from a variety of species. A gel filtration and/or an ion exchange step can be employed to further purify the immunoglobulin of interest.
Ion Exchange Chromatography SBCS-41
Subcloning of one positive clone to monoclonality by two rounds of limiting dilution. ELISA screening to detect positive antibody-secreting subclones. Cryopreservation of Ten (10) vials of the hybridoma cell line and isotyping of one monoclonal antibody
Protein A Purification SBCS-43
High yields of pure IgG can be obtained in a single-step purification using Protein A affinity chromatography, which differ in their ability to bind antibodies of different origins and IgG subclasses.
Protein G Purification SBCS-44
High yields of pure IgG can be obtained in a single-step purification using Protein G affinity chromatography, which differ in their ability to bind antibodies of different origins and IgG subclasses.
Size Exclusion/Gel Filtration Chromatography SBCS-42
A single gel matrix or a sequence of size exclusion columns can be used for purification of IgM and IgG antibodies. The purification strategy employed is dependent on the immunoglobulin class and the source of the material.
Low Endotoxin Purification SBCS-48
Purification under aseptic conditions to generate clean, low-endotoxin antibody preparations.
Purification of IgY from Chicken Egg Yolks SBCS-47
Note – Prior to initiating a protein purification project, the customer must provide SouthernBiotech with a signed assurance that any customer-supplied material is mycoplasma-free, non-infectious, non-carcinogenic, and has no known safety concerns to animals or humans.
To initiate a Custom Service Project -
Contact our Technical Services Department at 205.945.1774 or technical@southernbiotech.com
Toll-free within the US and Canada 800.722.2255
A project manager will be assigned and report to you regularly on an agreed schedule.
Discounts and third party arrangements are not accepted in conjunction with SouthernBiotech Custom Services.
All custom services are accepted and undertaken on a “best efforts” basis only. SouthernBiotech cannot and does not make any representations or warranties, expressed or implied, including any warranty of merchantability or fitness for any particular purpose for any material produced or service provided; and, all charges are due and payable as billed regardless of the outcome of our work.
Customer & Technical Service 800.722.2255 (USA & Canada) . 205.945.1774 . Fax 205.945.8768
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