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  • Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP

Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-HRP

Cat. No.:
6425-05
Donkey Anti-Goat IgG(H+L)-HRP antibody with minimal reactivity to human, mouse, rat, hamster, rabbit, chicken, horse, and guinea pig serum proteins for use in ELISA, western blot, and immunohistochemistry assays.
$145.00
Size Price (USD) Quantity
1.0 mL $145.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey IgG
Isotype Control 0175-05
Specificity Reacts with the heavy and light chains of goat IgG
Source Pooled antisera from donkeys hyperimmunized with goat IgG
Cross Adsorption Human, mouse, rat, hamster, rabbit, chicken, horse, and guinea pig serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Method Affinity chromatography on goat IgG covalently linked to agarose
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1
Western Blot 1
RRID Number AB_2796346

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

References and Citations

  1. 1. SouthernBiotech published data (IHC-FS, WB)

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  1. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6164-05).
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  2. Normal Donkey Serum
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  3. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Human SP ads-HRP (SB Cat. No. 6026-05).
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  4. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6157-05).
    Mouse Anti-Goat IgG Fc-HRP (SB115h)
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Mouse Anti-Goat IgG Fc-HRP (SB115d)
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  7. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6020-05).
    Rabbit F(ab')2 Anti-Goat IgG(H+L)-HRP
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  8. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Rabbit Anti-Goat IgG Fc-HRP (SB Cat. No. 6163-05).
    Rabbit Anti-Goat IgG Fc-HRP
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  9. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  10. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type IV Collagen-UNLB (SB Cat. No. 1340-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  11. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  12. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6160-05).
    Rabbit Anti-Goat IgG(H+L)-HRP
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  13. ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (
    Goat IgG Fc-UNLB
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  14. Paraffin embedded mouse spleen section was stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP (SB Cat. No. 6420-05), DAB, and hematoxylin.
    Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP
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  15. ELISA plate was coated with serially diluted Goat IgG Fab-UNLB (SB Cat. No. 0131-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fc-BIOT and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05)
    Goat IgG Fab-UNLB
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  16. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP (SB Cat. No. 6421-05).
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