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  • Goat Anti-Chicken IgY(H+L), Multi-Species SP ads-UNLB

Goat Anti-Chicken IgY(H+L), Multi-Species SP ads-UNLB

Cat. No.:
6105-01
Purified Goat Anti-Chicken IgY(H+L) antibody with minimal reactivity to human, mouse, rat, bovine, horse, hamster, goat, sheep, rabbit, and guinea pig serum proteins for use in ELISA assays.
$111.00
Size Price (USD) Quantity
1.0 mg $111.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with the heavy and light chains of chicken IgY
Source Pooled antisera from goats hyperimmunized with IgY
Cross Adsorption Human, mouse, rat, bovine, horse, hamster, goat, sheep, rabbit, and guinea pig serum proteins (SP); may react with immunoglobulins from other species and the light chains of other chicken immunoglobulins
Purification Method Affinity chromatography on chicken IgY covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796166

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  2. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF647 (SB Cat. No. 6425-31).
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  4. ELISA plate was coated with serially diluted Chicken IgY-UNLB (SB Cat. No. 0170-01). Immunoglobulin was detected with Goat Anti-Chicken IgY(H+L)-BIOT (SB Cat. No. 6100-08), Mouse Anti-Chicken IgM-BIOT (SB Cat. No. 8300-08), and Mouse Anti-Chicken IgA-BIOT (SB Cat. No. 8330-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  6. Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD3-UNLB (SB Cat. No. 8200-01; right) followed by Anti-Mouse IgG-HRP, DAB, and hematoxylin.
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  7. FLISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-PE (SB Cat. No. 6157-09).
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  8. Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD4-UNLB (SB Cat. No. 8210-01, right) followed by Anti-Mouse IgG-HRP, DAB, and hematoxylin.
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  9. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  10. Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD8α-UNLB (SB Cat. No. 8220-01; right) followed by Anti-Mouse IgG-HRP, DAB, and hematoxylin.
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  11. ELISA plate was coated with purified turkey IgY. Immunoglobulin was detected with Goat Anti-Turkey IgY(H+L)-UNLB (SB Cat. No. 6110-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  12. Normal chick cochlea was stained with phalloidin conjugated to AF546 and Mouse Anti-Chicken Monocyte/Macrophage-UNLB (SB Cat. No. 8420-01) followed by an AF488 secondary antibody.<br/>Image from Warchol ME, Schwendener RA, Hirose K. Depletion of resident macrophages does not alter sensory regeneration in the avian cochlea. PLoS One. 2012;7(12):e51574. Figure 1(b)<br/>Reproduced under the Creative Commons Attribution License
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