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  • Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-FITC

Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-FITC

Cat. No.:
1093-02
Goat Anti-Mouse IgG2b-FITC antibody with minimal reactivity to human, bovine, and horse serum proteins for use in western blot, immunocytochemistry, and other fluorescent assays.
$241.00
Size Price (USD) Quantity
1.0 mg $241.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with the heavy chain of mouse IgG2b
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Cross Adsorption Mouse IgG1, IgG2a, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2b covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 1
Western Blot 1
RRID Number AB_2794555
Gene ID 16016 (Mouse)
Gene ID Symbol Ighg2b (Mouse)
Gene ID Aliases IgG2b; Igh-3; gamma2b
UniProt ID P01867 (Mouse)
UniProt Name IGG2B_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

References and Citations

  1. 1. SouthernBiotech published data (ICC, WB)

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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>-FITC (SB Cat. No. 1091-02).
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>2b</sub>, Human ads-FITC (SB Cat. No. 1092-02) and DAPI.
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  6. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-AF647 (SB Cat. No. 1080-31).
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  7. Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
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  8. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-AF488 (SB Cat. No. 1100-30).
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  9. Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-FITC (SB Cat. No. 1090-02) and DAPI.
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  10. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45RA-UNLB followed by Rat Anti-Mouse IgG<sub>2b</sub>-FITC (SB Cat. No. 1186-02).
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  11. Wdpks1Δ-1 yeast cells treated with liquid nitrogen were stained with anti-melanin followed by Goat Anti-Mouse IgM, Human ads-TRITC (SB Cat. No. 1020-03).<br/>Image from Paolo WF Jr, Dadachova E, Mandal P, Casadevall A, Szaniszlo PJ, Nosanchuk JD. Effects of disrupting the polyketide synthase gene <i>WdPKS1</i> in <i>Wangiella</i> [<i>Exophiala</i>] <i>dermatitidis</i> on melanin production and resistance to killing by antifungal compounds, enzymatic degradation, and extremes in temperature. BMC Microbiol. 2006;6:55. Figure 5(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  12. Frozen section of TEPC 1165 cell line pellet was stained with Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07).<br/>Image from Wang H, Owens JD, Shih JH, Li M, Bonner RF, Mushinski JF. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics. 2006;7:97. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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