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Hamster Anti-Mouse CD69-LE/AF (H1.2F3)

Cat. No.:
Low Endotoxin/Azide Free Anti-Mouse CD69 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, costimulation, and activation assays.
Size Price (USD) Quantity
0.5 mg $325.00
More Information
Clone H1.2F3
Isotype Hamster (Armenian) IgG1
Isotype Control Hamster IgG-LE/AF
Specificity Mouse CD69
Alternative Names VEA, very early activation antigen, AIM, EA1, MLR3, gp34/28, Leu-23
Description CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. The monoclonal antibody H1.2F3 augments PMA-induced T-cell proliferation and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
Immunogen Mouse dendritic epidermal cell line Y245
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Costimulation – Reported in literature 1
Activation – Reported in literature 2

RRID Number AB_2795183
Gene ID 12515 (Mouse)
Gene ID Symbol Cd69 (Mouse)
Gene ID Aliases AIM; VEA; AI452015; 5830438K24Rik
UniProt ID P37217 (Mouse
UniProt Name CD69_MOUSE (Mouse)

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  1. 1. Yokoyama WM, Koning F, Kehn PJ, Pereira GM, Stingl G, Coligan JE, et al. Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation. J Immunol. 1988;141:369-76. (Immunogen, FC, IP, Costim)
  2. 2. Karlhofer FM, Yokoyama WM. Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways. J Immunol. 1991;146:3662-73. (FC, Activ)
  3. 3. Podd BS, Thoits J, Whitley N, Cheng H, Kudla KL, Taniguchi H, et al. T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice. J Immunol. 2006;176:6532-42. (IHC-FS)