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Mouse Anti-Human CD35-FITC (E11)

Cat. No.:
FITC Anti-Human CD35 antibody for use in flow cytometry, immunohistochemistry, and western blot assays.
Size Price (USD) Quantity
100 tests $196.00
More Information
Clone E11
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-FITC (15H6)
Specificity Human/Rhesus/Cynomolgus/Chimpanzee/Baboon CD35
Alternative Names CR1, complement receptor type 1, C3bR, C4bR, immune adherence receptor
Description The CD35 antigen is composed of four different allotypes having different molecular weights [A (or F) - 160 kDa, B (or S) - 190 kDa, C - 220 kDa, and D - 250 kDa]. It is the receptor for C3b and is found on B cells, a subset of T cells, monocytes, neutrophils, follicular dendritic cells, erythrocytes, and kidney glomelular podocytes. CD35 mediates neutrophil and monocyte phagocytosis of particles coated with C3b and/or C4b. This interaction also enhances phagocytosis mediated by the Fc receptors.
Immunogen Acute monocytic leukemia cells and normal blood monocytes
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Flow Cytometry – Quality tested 8,11
Immunohistochemistry-Frozen Sections – Reported in literature 7
Immunohistochemistry-Paraffin Sections – Reported in literature 6
Immunoprecipitation – Reported in literature 1,2
Western Blot – Reported in literature 3-5,9
ELISA – Reported in literature 1,2,4,5

RRID Number AB_2796996
Gene ID 1378 (Human)
718120 (Rhesus)
102117401 (Cynomolgus)
449643 (Chimpanzee)
101016492 (Baboon)
Gene ID Symbol CR1 (Human)
CR1 (Rhesus)
CR1 (Cynomolgus)
CR1 (Chimpanzee)
LOC101016492 (Baboon)
Gene ID Aliases C3BR; C4BR; CD35; KN; CK820_G0012775
UniProt ID P17927 (Human)
UniProt Name CR1_HUMAN (Human)

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  1. 1. Krych M, Hourcade D, Atkinson JP. Sites within the complement C3b/C4b receptor important for the specificity of ligand binding. Proc Natl Acad Sci USA. 1991;88:4353-7. (IP, ELISA)
  2. 2. Moulds JM, Nickells MW, Moulds JJ, Brown MC, Atkinson JP. The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. J Exp Med. 1991;173:1159-63. (IP, ELISA)
  3. 3. Dervillez X, Oudin S, Libyh MT, Tabary T, Reveil B, Philbert F, et al. Catabolism of the human erythrocyte C3b/C4b receptor (CR1, CD35): vesiculation and/or proteolysis?. Immunopharmacology. 1997;38:129-40. (WB)
  4. 4. Nickells M, Hauhart R, Krych M, Subramanian VB, Geoghegan-Barek K, Marsh HC Jr, et al. Mapping epitopes for 20 monoclonal antibodies to CR1. Clin Exp Immunol. 1998;112:27-33. (WB, ELISA)
  5. 5. Birmingham DJ, Gavit KF, McCarty SM, Yu CY, Rovin BH, Nagaraja HN, et al. Consumption of erythrocyte CR1 (CD35) is associated with protection against systemic lupus erythematosus renal flare. Clin Exp Immunol. 2005;143:274-80. (WB, ELISA)
  6. 6. Norton AJ, Isaacson PG. Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue. Am J Pathol. 1987;128:225-40. (IHC-PS)
  7. 7. Daridon C, Loddenkemper C, Spieckermann S, Kühl AA, Salama A, Burmester GR, et al. Splenic proliferative lymphoid nodules distinct from germinal centers are sites of autoantigen stimulation in immune thrombocytopenia. Blood. 2012;120:5021-31. (IHC-FS)
  8. 8. Yoshino N, Ami Y, Terao K, Tashiro F, Honda M. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim. 2000;49:97-110. (FC, Cynomolgus Reactivity)
  9. 9. Chen W, Logar CM, Shen X, Birmingham DJ. The chimpanzee and cynomolgus monkey erythrocyte immune adherence receptors are encoded by CR1-like genes. Immunogenetics. 2000;52:46-52. (WB, Cynomolgus & Chimpanzee Reactivity)
  10. 10. Birmingham DJ, Logar CM, Shen X, Chen W. The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element. J Immunol. 1996;157:2586-92. (WB, Baboon Reactivity)
  11. 11. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dörries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997;29:351-62. (FC, Rhesus Reactivity)
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