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Mouse Anti-Human CD45-SPRD (F10-89-4)

Cat. No.:
PE/CY5 (SPRD) Anti-Human CD45 antibody for use in flow cytometry assays.
Size Price (USD) Quantity
100 tests $253.00
More Information
Clone F10-89-4
Isotype Mouse IgG2aκ
Isotype Control Mouse IgG2a-SPRD (HOPC-1)
Specificity Human/Horse CD45
Alternative Names LCA, T200, Ly5
Description CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.
Immunogen Purified T cells from human lymph nodes
Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Trademark Information Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc.
Spectral Red® is a PE/CY5 tandem conjugate
Cy® is a registered trademark of Global Life Sciences Solutions Germany GmbH
Applications Flow Cytometry – Quality tested 1,5,9-11
Immunohistochemistry-Frozen Sections – Reported in literature 1-3
Immunocytochemistry – Reported in literature 4,5
Immunoprecipitation – Reported in literature 6,7
Western Blot – Reported in literature 7,8

RRID Number AB_2797029
Gene ID 5788 (Human)
100061950 (Horse)
Gene ID Symbol PTPRC (Human)
PTPRC (Horse)
Gene ID Aliases B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200
UniProt ID P08575 (Human
UniProt Name PTPRC_HUMAN (Human)

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  1. 1. Dalchau R, Kirkley J, Fabre JW. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980;10:737-44. (Immunogen, FC, IHC-FS)
  2. 2. Mason DY, Cordell JL, Abdulaziz Z, Naiem M, Bordenave G. Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies. J Histochem Cytochem. 1982;30:1114-22. (IHC-FS)
  3. 3. Salter DM, Krajewski AS, Dewar AE. Immunophenotype analysis of malignant histiocytosis of the intestine. J Clin Pathol. 1986;39:8-15. (IHC-FS)
  4. 4. Toscano MA, Bianco GA, Ilarregui JM, Croci DO, Correale J, Hernandez JD, et al. Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death. Nat Immunol. 2007;8:825-34. (ICC)
  5. 5. De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, et al. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 2012;81:312-23. (FC, ICC, Horse Reactivity)
  6. 6. Chen I, Chen H, Demetriou M. Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling. J Biol Chem. 2007;282:35361-72. (IP)
  7. 7. Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004;172:4298-307. (IP, WB)
  8. 8. Stillman BN, Hsu DK, Pang M, Brewer CF, Johnson P, Liu F, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol. 2006;176:778-89. (WB)
  9. 9. Young AJ, Holzgreve W, Dudler L, Schoeberlein A, Surbeck DV. Engraftment of human cord blood-derived stem cells in preimmune ovine fetuses after ultrasound-guided in utero transplantation. Am J Obstet Gynecol. 2003;189:698-701. (FC)
  10. 10. Mitchell P, Lee F, Hall C, Rigopoulos A, Smyth FE, Hekman A, et al. Targeting primary human Ph+ B-cell precursor leukemia-engrafted SCID mice using radiolabeled anti-CD19 monoclonal antibodies. J Nucl Med. 2003;44:1105-12. (FC)
  11. 11. Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived
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