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Mouse Anti-Human CD54-APC (15.2)

Cat. No.:
APC Anti-Human CD54 antibody for use in flow cytometry assays.
Size Price (USD) Quantity
100 tests $275.00
More Information
Clone 15.2
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-APC (15H6)
Specificity Human/Porcine CD54
Alternative Names ICAM-1, intracellular adhesion molecule-1, Ly-47
Description CD54, also known as intercellular cell adhesion molecule-1 (ICAM-1), is a 90 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It exhibits a wide tissue distribution being expressed on both hematopoietic and non-hematopoietic cells. ICAM-1 expression on leukocytes is low but is rapidly upregulated upon activation. Expression on endothelium and other non-hematopoietic cells is strongly upregulated by inflammatory mediators. The ligands of CD54 are CD11a/LFA-1α and CD11b/Mac-1α. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels particularly in areas of inflammation. CD54 on antigen-presenting cells (APC) contributes to antigen-specific T cell activation presumably by enhancing interactions between T cells and APC.
Immunogen Rheumatoid synovial cells and human monocytes
Conjugate APC (Allophycocyanin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Flow Cytometry – Quality tested 14-17
Immunohistochemistry-Frozen Sections – Reported in literature 1-3
Immunocytochemistry – Reported in literature 4,18
Immunoprecipitation – Reported in literature 7
Western Blot – Reported in literature 5
Blocking – Reported in literature 6,8,9
Neutralization – Reported in literature 7
ELISA – Reported in literature 10-13

RRID Number AB_2796800
Gene ID 3383 (Human)
396750 (Porcine)
Gene ID Symbol ICAM1 (Human)
ICAM1 (Porcine)
Gene ID Aliases BB2; CD54; P3.58; ICAM-1
UniProt ID P05362 (Human)
UniProt Name ICAM1_HUMAN (Human)

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  1. 1. Norton J, Sloane JP, Al-Saffar N, Haskard DO. Expression of adhesion molecules in human intestinal graft-versus-host disease. Clin Exp Immunol. 1992;87:231-6. (IHC-FS)
  2. 2. Bloom S, Simmons D, Jewell DP. Adhesion molecules intercellular adhesion molecule-1 (ICAM-1), ICAM-3 and B7 are not expressed by epithelium in normal or inflamed colon. Clin Exp Immunol. 1995;101:157-63. (IHC-FS)
  3. 3. Sousa AR, Lane SJ, Atkinson BA, Poston RN, Lee TH. The effects of prednisolone on the cutaneous tuberculin response in patients with corticosteroid-resistant bronchial asthma. J Allergy Clin Immunol. 1996;97:698-706. (IHC-FS)
  4. 4. Lin Y, Weisdorf DJ, Solovey A, Hebbel RP. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest. 2000;105:71-7. (ICC)
  5. 5. Grewe M, Stege H, Vink A, Klammer M, Ruzicka T, Roza L, et al. Inhibition of intercellular adhesion molecule-1 (ICAM-1) expression in ultraviolet B-irradiated human antigen-presenting cells is restored after repair of cyclobutane pyrimidine dimers. Exp Dermatol. 2000;9:423-30. (WB)
  6. 6. Berendt AR, McDowall A, Craig AG, Bates PA, Sternberg MJ, Marsh K, et al. The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site. Cell. 1992;68:71-81. (Block)
  7. 7. Rizzuto CD, Sodroski JG. Contribution of virion ICAM-1 to human immunodeficiency virus infectivity and sensitivity to neutralization. J Virol. 1997;71:4847-51. (IP, Neut)
  8. 8. Duperray A, Languino LR, Plescia J, McDowall A, Hogg N, Craig AG, et al. Molecular identification of a novel fibrinogen binding site on the first domain of ICAM-1 regulating leukocyte-endothelium bridging. J Biol Chem. 1997;272:435-41. (Block)
  9. 9. Porter JC, Falzon M, Hall A. Polarized localization of epithelial CXCL11 in chronic obstructive pulmonary disease and mechanisms of T cell egression. J Immunol. 2008;180:1866-77. (Block)
  10. 10. Chen K, Reece LM, Leary JF. Mitochondrial glutathione modulates TNF-α-induced endothelial cell dysfunction. Free Radic Biol Med. 1999;27:100-9. (ELISA)
  11. 11. Kalogeris TJ, Kevil CG, Laroux FS, Coe LL, Phifer TJ, Alexander JS. Differential monocyte adhesion and adhesion molecule expression in venous and arterial endothelial cells. Am J Physiol. 1999;276:L9-19. (ELISA)
  12. 12. Kalogeris TJ, Laroux FS, Cockrell A, Ichikawa H, Okayama N, Phifer TJ, et al. Effect of selective proteasome inhibitors on TNF-induced activation of primary and transformed endothelial cells. Am J Physiol. 1999;276(4):C856-64. (ELISA)
  13. 13. Chen K, Chang BH, Younan P, Shlykov SG, Sanborn BM, Chan L. Increased intracellular calcium transients by calmodulin antagonists differentially modulate tumor necrosis factor-α-induced E-selectin and ICAM-1 expression. Atherosclerosis. 2002;165:5-13. (ELISA)
  14. 14. Franken M, Devergne O, Rosenzweig M, Annis B, Kieff E, Wang F. Comparative analysis identifies conserved tumor necrosis factor receptor-associated factor 3 binding sites in the human and simian Epstein-Barr virus oncogene LMP1. J Virol. 1996;70:7819-26. (FC)
  15. 15. Yamazaki K, Spuill G, Rhoderick J, Spielman J, Savaraj N, Podack ER. Small cell lung carcinomas express shared and private tumor antigens presented by HLA-A1 or HLA-A2. Cancer Res. 1999;59:4642-50. (FC)
  16. 16. Varas A, Jiménez E, Sacedón R, Rodríguez-Mahou M, Maroto E, Zapata AG, et al. Analysis of the human neonatal thymus: evidence for a transient thymic involution. J Immunol. 2000;164:6260-7. (FC)
  17. 17. Hernández-Lopez C, Valencia J, Hidalgo L, Martínez VG, Zapata AG, Sacedón R, et al. CXCL12/CXCR4 signaling promotes human thymic dendritic cell survival regulating the Bcl-2/Bax ratio. Immunol Lett. 2008;120:72-8. (FC)
  18. 18. Edamura K, Nasu K, Iwami Y, Nishimura R, Ogawa H, Sasaki N, et al. Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells. Xenotransplantation. 2005;12:327-32. (ICC, Porcine Reactivity)
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