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Mouse Anti-Human CD74-LE/AF (LN2)

Cat. No.:
Low Endotoxin/Azide Free Anti-Human CD74 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, immunoprecipitation, and blocking assays.
Size Price (USD) Quantity
0.5 mg $330.00
More Information
Clone LN2
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-LE/AF (15H6)
Specificity Human CD74
Alternative Names Invariant chain, Ii, class II-specific chaperone
Description CD74 represents a type II integral membrane protein known as MHC class II-associated invariant chain. It is moderately expressed on B cells and weakly on monocytes and macrophages. CD74 is also found on a subset of activated T cells, Langerhans cells, dendritic cells, and some epithelial cells. CD74 may play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface.
Immunogen Cell line SU-DHL-4 nuclei
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications Flow Cytometry – Quality tested 4,6,10,11
Immunohistochemistry-Frozen Sections – Reported in literature 1,2
Immunohistochemistry-Paraffin Sections – Reported in literature 1,3-5
Immunocytochemistry – Reported in literature 1,6
Immunoprecipitation – Reported in literature 1,6
Western Blot – Reported in literature 7
Blocking – Reported in literature 8,9

RRID Number AB_2797095
Gene ID 972 (Human)
Gene ID Symbol CD74 (Human)
Gene ID Aliases DHLAG; HLADG; II; Ia-GAMMA; p33
UniProt ID P04233 (Human
UniProt Name HG2A_HUMAN (Human)

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  1. 1. Epstein AL, Marder RJ, Winter JN, Fox RI. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984;133:1028-36. (Immunogen, IHC-FS, IHC-PS, ICC, IP)
  2. 2. Pack M, Trumpfheller C, Thomas D, Park CG, Granelli-Piperno A, Münz C, et al. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2007;123:438-46. (IHC-FS)
  3. 3. Sarker AB, Akagi T, Jeon HJ, Miyake K, Murakami I, Yoshino T, et al. Bauhinia purpurea--a new paraffin section marker for Reed-Sternberg cells of Hodgkin's disease. A comparison with Leu-M1 (CD15), LN2 (CD74), peanut agglutinin, and Ber-H2 (CD30). Am J Pathol. 1992;141:19-23. (IHC-PS)
  4. 4. Burton JD, Ely S, Reddy PK, Stein R, Gold DV, Cardillo TM, et al. CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res. 2004;10:6606-11. (IHC-PS, FC)
  5. 5. Sepp N, Radaszkiewicz T, Meijer CJ, Smolle J, Seewann H, Fritsch P, et al. Specific skin manifestations in acute leukemia with monocytic differentiation. A morphologic and immunohistochemical study of 11 cases. Cancer. 1993;71:124-32. (IHC-PS)
  6. 6. Triantafilou K, Triantafilou M, Wilson KM, Cherry RJ, Fernandez N. Intracellular and cell surface heterotypic associations of human leukocyte antigen-DR and human invariant chain. Hum Immunol. 1999;60:1101-12. (ICC, IP, FC)
  7. 7. Imam A, Taylor CR, inventors; University of Southern California, assignee. Antigen specifically expressed on the surface of B cells and Hodgkin's cells. United States patent US 5304635. 1994 Apr 19. (WB)
  8. 8. Zhou C, Lu L, Tan S, Jiang S, Chen Y. HIV-1 glycoprotein 41 ectodomain induces activation of the CD74 protein-mediated extracellular signal-regulated kinase/mitogen-activated protein kinase pathway to enhance viral infection. J Biol Chem. 2011;286:44869-77. (Block)
  9. 9. Klasen C, Ohl K, Sternkopf M, Schachar I, Schmitz C, Heussen N, et al. MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling. J Immunol. 2014;192:5273-84. (Block)
  10. 10. Aiello A, Delia D, Borrello MG, Biassoni D, Giardini R, Fontanella E, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992;13:502-9. (FC)
  11. 11. Butrym A, Majewski M, Dzietczenia J, Kuliczkowski K, Mazur G. High CD74 expression correlates with ZAP70 expression in B cell chronic lymphocytic leukemia patients. Med Oncol. 2013;30:560. (FC)
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