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  • Mouse Anti-Human Kappa-BIOT (SB81a - Recombinant)

Mouse Anti-Human Kappa-BIOT (SB81a - Recombinant)

Cat. No.:
29230-08
Biotin Anti-Human Kappa recombinant antibody for use in ELISA and flow cytometry assays.
$116.00
Size Price (USD) Quantity
0.25 mg $116.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Clone SB81a-R
(Genetically derived from DNA sequences of a parental hybridoma clone; for details on the parental clone, see Cat. No. 9230)
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-BIOT (15H6)
Specificity Human Kappa
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.5 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested
Flow Cytometry – Quality tested

RRID Number AB_2796705
Gene ID 3514 (Human)
Gene ID Symbol IGKC (Human)
Gene ID Aliases HCAK1D; Km; IGKC
UniProt ID P01834 (Human)
UniProt Name IGKC_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-UNLB (SB Cat. No. 1080-01), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG<sub>1</sub>-BIOT (SB Cat. No. 0102-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  2. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  3. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Lambda-BIOT (Recombinant) - (SB Cat. No. 29180-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05).
    Mouse Anti-Human Lambda-BIOT (JDC-12-R - Recombinant)
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  4. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-BIOT (SB Cat. No. 2060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human Kappa-BIOT
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  7. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  8. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-BIOT (SB Cat. No. 2070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  9. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  10. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-BIOT (SB Cat. No. 2061-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
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  12. CD19+ human B-lymphocytes were stained with Goat Anti-Human Lambda, Mouse ads-BIOT (SB Cat. 2071-08) and Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-PE (SB Cat. No. 2063-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  13. ELISA plate was coated with serially diluted Human IgA Kappa-UNLB (SB Cat. No. 0155K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgA Kappa-UNLB
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  14. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-BIOT (SB Cat. No. 2073-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  15. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-BIOT (SB Cat. No. 2062-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  16. Goat F(ab')2 Anti-Human Kappa, Mouse ads-BIOT
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