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Mouse IgG1-AF700 (15H6)

Cat. No.:
Mouse IgG1-Alexa Fluor® 700 for use as a control in flow cytometry assays.
Size Price (USD) Quantity
100 tests $196.00
More Information
Clone 15H6
Isotype Mouse IgG1κ
Specificity T-2 mycotoxin
Conjugate AF700 (Alexa Fluor® 700)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 5-10,12,14
ELISA – Quality tested 1-4
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 11,12
Immunohistochemistry-Paraffin Sections – Reported in literature 13,23
Immunocytochemistry – Reported in literature 14,15
Western Blot – Reported in literature 16,17
Blocking – Reported in literature 8
In vitro Control – Reported in literature 5,7,12,14,18
In vivo Control – Reported in literature 19-21
Multiplex – Reported in literature 24

RRID Number AB_2793861

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  1. 1. Fritz JH, Rojas OL, Simard N, McCarthy DD, Hapfelmeirer S, Rubino S, et al. Acquisition of a multifunctional IgA+ plasma cell phenotype in the gut. Nature. 2012;481:199-203. (ELISA)
  2. 2. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
  3. 3. DiLillo DJ, Tan GS, Palese P, Ravetch JV. Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo. Nat Med. 2014;20:143-51. (ELISA)
  4. 4. Halemano K, Guo K, Heilman KJ, Barrett BS, Smith DS, Hasenkrug KJ, et al. Immunoglobulin somatic hypermutation by APOBEC3/Rfv3 during retroviral infection. Proc Natl Acad Sci USA. 2014;111:7759-64. (ELISA)
  5. 5. Charerntantanakul W, Platt R, Roth JA. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61. (FC, in vitro control)
  6. 6. Zurney J, Howard KE, Sherry B. Basal expression levels of IFNAR and Jak-STAT components are determinants of cell-type-specific differences in cardiac antiviral responses. J Virol. 2007;81:13668-80. (FC)
  7. 7. Chua W, Kim S, Myers N, Huang S, Yu L, Fremont DH, et al. Endogenous MHC-related protein 1 is transiently expressed on the plasma membrane in a conformation that activates mucosal-associated invariant T cells. J Immunol. 2011;186:4744-50. (FC, in vitro control)
  8. 8. Sinka L, Biasch K, Khazaal I, Péault B, Tavian M. Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo. Blood. 2012;119:3712-23. (FC, Block)
  9. 9. Troyer RM, Thompson J, Elder JH, VandeWoude S. Accessory genes confer a high replication rate to virulent feline immunodeficiency virus. J Virol. 2013;87:7940-51. (FC)
  10. 10. Weckbach LT, Gola A, Winkelmann M, Jakob SM, Groesser L, Borgolte J, et al. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via β2 integrins (CD11/CD18). Blood. 2014;123:1887-96. (FC)
  11. 11. Kiekens RC, Thepen T, Bihari IC, Knol EF, Van De Winkel JG, Bruijnzeel-Koomen CA. Expression of Fc receptors for IgG during acute and chronic cutaneous inflammation in atopic dermatitis. Br J Dermatol. 2000;142:1106-13. (IHC-FS)
  12. 12. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC, IHC-FS, in vitro control)
  13. 13. Roberts S, Kosanke S, Dunn ST, Jankelow D, Duran CM, Cunningham MW. Pathogenic mechanisms in rheumatic carditis: focus on valvular endothelium. J Infect Dis. 2001;183:507-11. (IHC-PS)
  14. 14. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC, ICC, in vitro control)
  15. 15. Sleeman KE, Kendrick H, Ashworth A, Isacke CM, Smalley MJ. CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Res. 2006;8:R7. (ICC)
  16. 16. Howell MD, Kim BE, Gao P, Grant AV, Boguniewicz M, DeBenedetto A, et al. Cytokine modulation of atopic dermatitis filaggrin skin expression. J Allergy Clin Immunol. 2007;120:150-5. (WB)
  17. 17. Lucas CR, Cordero-Nieves HM, Erbe RS, McAlees JW, Bhatia S, Hodes RJ, Campbell KS, et al. Prohibitins and the cytoplasmic domain of CD86 cooperate to mediate CD86 signaling in B lymphocytes. J Immunol. 2013;190:723-36. (WB)
  18. 18. Hsu H, Baldwin CL, Telfer JC. The endocytosis and signaling of the γδ T cell coreceptor WC1 are regulated by a dileucine motif. J Immunol. 2015;194:2399-406. (In vitro control)
  19. 19. Gong J, Yang DJ, Kohanim S, Angelo LS, Kurzrock R. Molecular imaging of gefitinib activity in an epidermal growth factor receptor (EGFR)-bearing xenograft model. Cancer Biol Ther. 2009;8:2237-45. (In vivo control)
  20. 20. Xie F, Sun D, Schering A, Nakao S, Zandi S, Liu P, et al. Novel molecular imaging approach for subclinical detection of iritis and evaluation of therapeutic success. Am J Pathol. 2010;177:39-48. (In vivo control)
  21. 21. Sun D, Nakao S, Xie F, Zandi S, Bagheri A, Rezaei Kanavi M, et al. Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis. FASEB J. 2014;28:3942-51. (In vivo control)
  22. 22. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control)
  23. 23. French KJ, Coatney RW, Renninger JP, Hu CX, Gales TL, Zhao S, et al. Differences in effects on myocardium and mitochondria by angiogenic inhibitors suggest separate mechanisms of cardiotoxicity. Toxicol Pathol. 2010;38:691-702. (IHC-PS)
  24. 24. Hönger G, Hopfer H, Arnold M, Spriewald BM, Schaub S, Amico P. Pretransplant IgG subclasses of donor-specific human leukocyte antigen antibodies and development of antibody-mediated rejection. Transplantation. 2011;92:41-7. (Multiplex)
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