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Mouse IgG2a-UNLB (HOPC-1)

Cat. No.:
Purified Mouse IgG2a for use as a control in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, immunoprecipitation, and in vitro assays and as a standard in ELISA.
Size Price (USD) Quantity
1.0 mg $270.00
More Information
Clone HOPC-1
Isotype Mouse IgG2aλ
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 6-12
ELISA – Quality tested 1-5
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 13,14
Immunohistochemistry-Paraffin Sections 20
Immunocytochemistry – Reported in literature 15,21
Western Blot – Reported in literature 17
Immunoprecipitation – Reported in literature 15,16
In vitro Control – Reported in literature 7,8,17
In vivo Control – Reported in literature 17,18

RRID Number AB_2736850

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  1. 1. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)
  2. 2. Wang J, Anders RA, Wu Q, Peng D, Cho JH, Sun Y, et al. Dysregulated LIGHT expression on T cells mediates intestinal inflammation and contributes to IgA nephropathy. J Clin Invest. 2004;113:826-35. (ELISA)
  3. 3. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA)
  4. 4. Kamphuis T, Meijerhof T, Stegmann T, Lederhofer J, Wilschut J, de Haan A. Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice. PLoS One. 2012;7(5):e36812. (ELISA)
  5. 5. Faustino L, Fonseca DM, Florsheim EB, Resende RR, Lepique AP, Faquim-Mauro E, et al. Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation. Mucosal Immunol. 2014;7:1199-208. (ELISA)
  6. 6. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (FC)
  7. 7. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (FC, in vitro control)
  8. 8. Cosmi L, Liotta F, Lazzeri E, Francalanci M, Angeli R, Mazzinghi B, et al. Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes. Blood. 2003;102:4107-14. (FC, in vitro control)
  9. 9. Charerntantanakul W, Platt R, Roth JA. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61. (FC)
  10. 10. Crescioli C, Cosmi L, Borgogni E, Santarlasci V, Gelmini S, Sottili M, et al. Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes. J Endocrinol. 2007;195:145-55. (FC)
  11. 11. Wen K, Bui T, Li G, Liu F, Li Y, Kocher J, et al. Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection. Comp Immunol Microbiol Infect Dis. 2012;35:289-301. (FC)
  12. 12. Kroesen M, Nierkens S, Ansems M, Wassink M, Orentas RJ, Boon L, et al. A transplantable TH-MYCN transgenic tumor model in C57Bl/6 mice for preclinical immunological studies in neuroblastoma. Int J Cancer. 2014;134:1335-45. (FC)
  13. 13. Chen M, Lam BK, Kanaoka Y, Nigrovic PA, Audoly LP, Austen KF, et al. Neutrophil-derived leukotriene B4 is required for inflammatory arthritis. J Exp Med. 2006;203:837-42. (IHC-FS)
  14. 14. Balogh A, Pozsgay J, Matkó J, Dong Z, Kim CJ, Várkonyi T, et al. Placental protein 13 (PP13/galectin-13) undergoes lipid raft-associated subcellular redistribution in the syncytiotrophoblast in preterm preeclampsia and HELLP syndrome. Am J Obstet Gynecol. 2011;205:156e1-14. (IHC-FS)
  15. 15. Guimarães AJ, Hamilton AJ, de M Guedes HL, Nosanchuk JD, Zancopé-Oliveira RM. Biological function and molecular mapping of M antigen in yeast phase of Histoplasma capsulatum. PLoS One. 2008;3(10):e3449. (ICC, IP)
  16. 16. Lindner JM, Kayo H, Hedlund S, Fukuda Y, Fukao T, Nielsen PJ. Cutting edge: The transcription factor Bob1 counteracts B cell activation and regulates miR-146a in B cells. J Immunol. 2014;192:4483-6. (IP)
  17. 17. Lighvani S, Baik N, Diggs JE, Khaldoyanidi S, Parmer RJ, Miles LA. Regulation of macrophage migration by a novel plasminogen receptor Plg-RKT. Blood. 2011;118:5622-30. (WB, in vitro control, in vivo control)
  18. 18. Smith MA, Weingarten RA, Russo LM, Ventura CL, O'Brien AD. Antibodies against hemolysin and cytotoxic necrotizing factor type 1 (CNF1) reduce bladder inflammation in a mouse model of urinary tract infection with toxigenic uropathogenic Escherichia coli. Infect Immun. 2015;83:1661-73. (In vivo control)
  19. 19. Xia Y, Janda A, Eryilmaz E, Casadevall A, Putterman C. The constant region affects antigen binding of antibodies to DNA by altering secondary structure. Mol Immunol. 2013;56:28-37. (Circular dichroism spectroscopy protein control)
  20. 20. SouthernBiotech published data (IHC-PS)
  21. 21. Pilling D, Tucker NM, Gomer RH. Aggregated IgG inhibits the differentiation of human fibrocytes. J Leukoc Biol. 2006;79:1242-51 (ICC)
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