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  • Donkey F(ab')2 Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP

Donkey F(ab')2 Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP

Cat. No.:
6421-05
Donkey F(ab')2 Anti-Goat IgG(H+L)-HRP antibody with minimal reactivity to mouse and rat serum proteins for use in ELISA assays.
$168.00
Size Price (USD) Quantity
1.0 mL $168.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of goat IgG
Source Pepsin digest of Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads (SB Cat. No. 6420)
Cross Adsorption Mouse and rat serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Method Gel filtration chromatography of pepsin digested antibody
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796344

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified mouse IgG Fc and IgG Fab and human, bovine, and horse serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG Fc, Human/Bovine/Horse SP ads-UNLB (SB Cat. No. 1013-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  9. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  12. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6160-05).
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  13. ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (
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  14. Paraffin embedded mouse spleen section was stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-HRP (SB Cat. No. 6420-05), DAB, and hematoxylin.
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  15. ELISA plate was coated with serially diluted Goat IgG Fab-UNLB (SB Cat. No. 0131-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fc-BIOT and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05)
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