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  • Goat Anti-Mouse IgG2c, Human/Bovine/Horse SP ads-AF555

Goat Anti-Mouse IgG2c, Human/Bovine/Horse SP ads-AF555

Cat. No.:
1077-32
Goat Anti-Mouse IgG2c-Alexa Fluor® 555 antibody with minimal reactivity to human, bovine, and horse serum proteins for use in fluorescent assays.
$411.00
Size Price (USD) Quantity
1.0 mg $411.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-32
Specificity Reacts with the heavy chain of C57BL/6 mouse IgG2c
Source Pooled antisera from goats hyperimmunized with mouse IgG2c
Cross Adsorption Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2c covalently linked to agarose
Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2794456
Gene ID 404711 (Mouse)
Gene ID Symbol Ighg2c (Mouse)
Gene ID Aliases Igh-1b

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with serially diluted Mouse IgG<sub>2c</sub>-UNLB (SB Cat. No. 0122-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-BIOT (SB Cat. No. 1079-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgG2c-UNLB (6.3)
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>-BIOT (SB Cat. No. 1078-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2c-BIOT
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  4. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-FITC (SB Cat. No. 1080-02).
    Goat Anti-Mouse IgG2a, Human ads-FITC
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
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  6. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-AF647 (SB Cat. No. 1090-31).
    Goat Anti-Mouse IgG2b, Human ads-AF647
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  7. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-AF488 (SB Cat. No. 1100-30).
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  8. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-AF555 (SB Cat. No. 1079-32).
    Goat Anti-Mouse IgG2c, Human ads-AF555
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  9. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-AF647 (SB Cat. No. 1070-31).
    Goat Anti-Mouse IgG1, Human ads-AF647
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  10. Wdpks1Δ-1 yeast cells treated with liquid nitrogen were stained with anti-melanin followed by Goat Anti-Mouse IgM, Human ads-TRITC (SB Cat. No. 1020-03).<br/>Image from Paolo WF Jr, Dadachova E, Mandal P, Casadevall A, Szaniszlo PJ, Nosanchuk JD. Effects of disrupting the polyketide synthase gene <i>WdPKS1</i> in <i>Wangiella</i> [<i>Exophiala</i>] <i>dermatitidis</i> on melanin production and resistance to killing by antifungal compounds, enzymatic degradation, and extremes in temperature. BMC Microbiol. 2006;6:55. Figure 5(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
    Goat Anti-Mouse IgM, Human ads-TRITC
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  11. ELISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-BIOT (SB Cat. No. 1110-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgE-BIOT
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