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Hamster Anti-Mouse CD103-LE/AF (2E7)

Cat. No.:
1810-14
Low Endotoxin/Azide Free Anti-Mouse CD103 antibody for use in flow cytometry, immunohistochemistry, immunoprecipitation, and activation assays.
$270.00
Size Price (USD) Quantity
0.5 mg $270.00
More Information
Clone 20000000
Isotype Hamster (Armenian) IgG2
Isotype Control Hamster IgG-LE/AF
Specificity Mouse CD103
Alternative Names Integrin αIEL, αE integrin, ITGAE
Description CD103 is a member of the integrin series of adhesion molecules. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis, and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 monoclonal antibody M290. 2E7 is reported to have signal-inducing activity in a redirected lysis assay and to costimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro which runs counter to the usual inhibition of CTL by anti-integrins. This costimulatory activity is also shared by the monoclonal antibody M290. The property of costimulation is not unique to the mouse since the combination of anti-human αIEL plus anti-TCR will produce similar proliferation in human cells with the exception that CD8+ cells are not stimulated.
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications Flow Cytometry – Quality tested 2
Immunoprecipitation – Reported in literature 2
Immunohistochemistry-Frozen Sections – Reported in literature 2
Activation – Reported in literature 3

RRID Number AB_2795380
Gene ID 16407 (Mouse)
Gene ID Symbol Itgae (Mouse)
Gene ID Aliases A530055J10; CD103; aM290; alpha-E1; alpha-M290
UniProt ID Q60677 (Mouse)
UniProt Name ITAE_MOUSE (Mouse)

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  1. 1. Goodman T, Lefrancois L. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989;170:1569-81. (Immunogen)
  2. 2. Lefrançois L, Barrett TA, Havran WL, Puddington L. Developmental expression of the αIELβ7 integrin on T cell receptor γδ and T cell receptor αβ T cells. Eur J Immunol. 1994;24:635-40. (IP, IHC-FS, FC)
  3. 3. Müller S, Jungo M, Aichele P, Mueller C. CD5- CD8αβ intestinal intraepithelial lymphocytes (IEL) are induced to express CD5 upon antigen-specific activation: CD5- and CD5+ CD8αβ IEL do not represent separate T cell lineages. Eur J Immunol. 1997;27:1756-61. (Activ)