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Mouse Anti-Chicken CD8α-PE (EP72)

Cat. No.:
PE Anti-Chicken CD8α antibody for use in flow cytometry assays.
Size Price (USD) Quantity
0.1 mg $237.00
More Information
Clone EP72
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-PE (A-1)
Specificity Chicken CD8α
Description In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCRγδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRγδ CD8αβ cells in the chicken that is not found in rodents. The monoclonal antibody EP72 recognizes the CD8α chain.
Immunogen Chicken splenocytes
Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration 0.1 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Flow Cytometry – Quality tested 4-7
Immunohistochemistry-Frozen Sections – Reported in literature 2,3

RRID Number AB_2796540
Gene ID 403158 (Chicken)
Gene ID Symbol CD8A (Chicken)
Gene ID Aliases CD8

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  1. 1. Paramithiotis E, Tkalec L, Ratcliffe MJ. High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes. J Immunol. 1991;147:3710-7. (Immunogen)
  2. 2. Reemers SS, van Haarlem D, Groot Koerkamp MJ, Vervelde L. Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow. J Gen Virol. 2009;90:2134-46. (IHC-FS)
  3. 3. Reemers SS, Jansen C, Groot Koerkamp MJ, van Haarlem D, van de Haar P, Degen WG, et al. Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: a transcriptomics analysis of adjuvanted vaccines. Vaccine. 2010;28:6351-60. (IHC-FS)
  4. 4. Marmor MD, Benatar T, Ratcliffe MJ. Retroviral transformation in vitro of chicken T cells expressing either α/β or γ/δ T cell receptors by reticuloendotheliosis virus strain T. J Exp Med. 1993;177:647-56. (FC)
  5. 5. Choi KD, Lillehoj HS, Song KD, Han JY. Molecular and functional characterization of chicken IL-15. Dev Comp Immunol. 1999;23:165-77. (FC)
  6. 6. Chen KL, Tsay SM, Chiou PW, Sun CP, Weng BC. Effects of caponization and different forms of exogenous androgen implantation on immunity in male chicks. Poult Sci. 2010;89:887-94. (FC)
  7. 7. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. (FC)
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