Mouse Anti-Human Fas Ligand-FITC (SB93a)
FITC Anti-Human Fas Ligand antibody for use in flow cytometry, immunohistochemistry, and western blot assays.
|Isotype||Mouse (BALB/c) IgG2bκ|
|Isotype Control||Mouse IgG2b-FITC (A-1)|
|Specificity||Human/Chicken Fas ligand|
|Alternative Names||FasL, CD178, CD95L, TNFSF6|
Fas ligand (CD178) is a 40 kD type II transmembrane protein that is a member of the tumor necrosis factor superfamily. This well characterized potent apoptotic factor is utilized by cytotoxic T cells and natural killer cells to selectively kill virus infected and tumorigenic cells. Moreover, studies indicate that Fas ligand is an important regulator in immune homeostasis where it has been shown to down-regulate immune responses during activation-induced cell death. Fas ligand initiates apoptosis by binding to its receptor, CD95, on target cells which facilitates recruitment of numerous signaling proteins to form a death inducing signaling complex. This signaling complex is then believed to propagate the apoptotic signal through the recruitment and activation of caspase-8.
The SB93a monoclonal antibody detects a band at ~40 kDa corresponding to the transmembrane/insoluble form of Fas ligand.
|Immunogen||Recombinant human Fas ligand|
|Buffer Formulation||Phosphate buffered saline containing < 0.1% sodium azide|
|Recommended Storage||2-8°C; Avoid exposure to light|
Flow Cytometry – Quality tested 1-3|
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunofluorescence – Reported in literature 5
Western Blot 6
|Gene ID Symbol||
|Gene ID Aliases||ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; TNFSF6; TNLG1A|
- 1. Carney EF, Srinivasan V, Moss PA, Taylor AM. Classical ataxia telangiectasia patients have a congenitally aged immune system with high expression of CD95. J Immunol. 2012;189:261-8. (FC)
- 2. Goldberg J, Bier C, Hotz-Behofsits C, Hanscom S, inventors; PIN Pharma, Inc., assignee. Treatment of inflammation, autoimmune, and neurodegenerative disorders with immunosuppressive tat derivative polypeptides. United States patent application publication US 2014/0178420 A1. 2014 Jun 26. (FC)
- 3. Mensen A, Oh Y, Becker SC, Hemmati PG, Jehn C, Westermann J, et al. Apoptosis susceptibility prolongs the lack of memory B cells in acute leukemic patients after allogeneic hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2015;21:1895-906. (FC)
- 4. Rauf A, Khatri M, Murgia MV, Saif YM. Fas/FasL and perforin-granzyme pathways mediated T cell cytotoxic responses in infectious bursal disease virus infected chickens. Results Immunol. 2012;2:112-9. (IHC-FS, Chicken Reactivity)
- 5. Yunker PJ, Asahara H, Hung K, Landry C, Arriage LR, Akartuna I. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci USA. 2016;113:608-13. (IF)
- 6. SouthernBiotech unpublished data
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