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  • Rabbit Anti-Goat IgG Fab-BIOT

Rabbit Anti-Goat IgG Fab-BIOT

Cat. No.:
6022-08
Rabbit Anti-Goat IgG Fab-Biotin antibody for use in ELISA assays.
$208.00
Size Price (USD) Quantity
0.5 mg $208.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit IgG
Isotype Control Rabbit IgG-BIOT
Specificity Reacts with the Fab region of goat IgG
Source Pooled antisera from rabbits hyperimmunized with goat IgG
Cross Adsorption Goat IgG Fc; may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Method Affinity chromatography on goat IgG Fab covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA

RRID Number AB_2796104

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with Goat Anti-Rabbit IgG-UNLB (SB Cat. No. 4030-01) and Goat Anti-Rabbit IgM-UNLB (SB Cat. No. 4020-01). Serially diluted Rabbit IgG-BIOT (SB Cat. No. 0111-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Rabbit IgG-BIOT
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  2. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  4. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115h)
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  5. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
    Mouse Anti-Biotin-PE (SB58c)
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  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6158-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115d)
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  8. Paraffin embedded rat kidney section post 30% blood volume loss and treatment with ketoprofen was stained with anti-IL-1 followed by Rabbit Anti-Goat IgG(H+L)-BIOT (SB Cat. No. 6160-08), HRP conjugated streptavidin, DAB, and hematoxylin.<br>Image from Guedes FS Jr, Cruz DS, Rodrigues MM, Silva LM, Amorim RL, Vianna PT, et al. Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect. Acta Cir Bras. 2012;27:37-42. Figure 3
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  9. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
    Streptavidin-AP
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  10. Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Rabbit Anti-Goat IgG(H+L), Human SP ads-BIOT (SB Cat. No. 6164-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
    Rabbit Anti-Goat IgG(H+L), Human SP ads-BIOT
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  11. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
    Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT
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  12. ELISA plate was coated with serially diluted Goat IgG Fab-UNLB (SB Cat. No. 0131-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fc-BIOT and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05)
    Goat IgG Fab-UNLB
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  13. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT (SB Cat. No. 6420-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT
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  14. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
    Goat IgG-UNLB
    0109-01
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  15. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT
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  16. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat F(ab')2 IgG-UNLB
    0110-01
    $201.00