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Rat Anti-Human IL-4-LE/AF (MP4-25D2)

Cat. No.:
Low Endotoxin/Azide Free Anti-Human IL-4 antibody for use in flow cytometry, immunohistochemistry, ELISA, ELISpot, and neutralization assays.
Size Price (USD) Quantity
0.5 mg $275.00
More Information
Clone MP4-25D2
Isotype Rat IgG1κ
Isotype Control Rat IgG1-LE/AF (KLH/G1-2-2)
Specificity Human/Rhesus/Cynomolgus/Baboon IL-4
Alternative Names Interleukin-4, T cell growth factor-2, TCGF-2 , Ia inducing factor, IaIF, B cell stimulating factor-1, BSF-1, Hodgkin's cell growth factor, HCGF, mast cell growth factor-2, MCGF-2, macrophage fusion factor, MFF, T cell growth factor-2, TCGF-2, Hodgkin's cell growth factor, HCGF
Immunogen CHO-expressed human IL-4
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications ELISA-Detection – Quality tested 1,2,4,5
ELISA-Capture – Reported in literature 3
ELISpot-Capture – Reported in literature 2,6
ELISpot-Detection – Reported in literature 7
Flow Cytometry – Reported in literature 8-10
Immunohistochemistry-Frozen Sections – Reported in literature 11-13
Neutralization – Reported in literature 1,14,15
Multiplex-Detection – Reported in literature 16

RRID Number AB_2794151
Gene ID 3565 (Human)
574281 (Rhesus)
107130068 (Cynomolgus)
100126747 (Baboon)
Gene ID Symbol IL4 (Human)
IL4 (Rhesus)
IL4 (Cynomolgus)
IL4 (Baboon)
Gene ID Aliases BCGF-1; BCGF1; BSF-1; BSF1; IL-4; EGK_16838
UniProt ID P05112 (Human)
P51492 (Rhesus)
P79339 (Cynomolgus)
Q865Y0 (Baboon)
UniProt Name IL4_HUMAN (Human)
IL4_MACMU (Rhesus)
IL4_MACFA (Cynomolgus)
IL4_PAPAN (Baboon)

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  1. 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
  2. 2. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection, ELISPOT-Capture)
  3. 3. Nyindo M, Kariuki TM, Mola PW, Farah IO, Elson L, Blanton RE, et al. Role of adult worm antigen-specific immunoglobulin E in acquired immunity to Schistosoma mansoni infection in baboons. Infect Immun. 1999;67:636-42. (ELISA-Capture, Baboon Reactivity)
  4. 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
  5. 5. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Detection)
  6. 6. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
  7. 7. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISPOT-Detection)
  8. 8. Koshiba M, Rosin DL, Hayashi N, Linden J, Sitkovsky MV. Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999;55:614-24. (FC)
  9. 9. Bandeira-Melo C, Sugiyama K, Woods LJ, Weller PF. Cutting edge: eotaxin elicits rapid vesicular transport-mediated release of preformed IL-4 from human eosinophils. J Immunol. 2001;166:4813-7. (FC)
  10. 10. Gansuvd B, Goodwin J, Asiedu CK, Jiang XL, Jargal U, Andrades P, et al. Invariant natural killer T cells from rhesus macaque spleen and peripheral blood are phenotypically and functionally distinct populations. J Med Primatol. 2008;37:1-11. (FC, Rhesus Reactivity)
  11. 11. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
  12. 12. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
  13. 13. Sparrelid E, Emanuel D, Fehniger T, Andersson U, Andersson J. Interstitial pneumonitis in bone marrow transplant recipients is associated with local production of TH2-type cytokines and lack of T cell-mediated cytotoxicity. Transplantation. 1997;63:1782-9. (IHC-FS)
  14. 14. Punnonen J, Yssel H, de Vries JE. The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells. J Allergy Clin Immunol. 1997;100:792-801. (Neut)
  15. 15. Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (Neut)
  16. 16. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Detection, Cynomolgus Reactivity)
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