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Rat Anti-Mouse CD5-AF647 (53-7.3)

Cat. No.:
1547-31
Alexa Fluor® 647 Anti-Mouse CD5 antibody for use in flow cytometry and immunohistochemistry assays.
$199.00
Size Price (USD) Quantity
0.1 mg $199.00
More Information
Clone 53-7.3
Isotype Rat (LOU/Ws1/M) IgG2aκ
Isotype Control Rat IgG2a-AF647 (KLH/G2a-1-1)
Specificity Mouse CD5
Alternative Names Lyt-1, Ly-1, Lymphocyte antigen 1, Ly-12, Ly-A, T1, Tp67
Description CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.
Immunogen Spleen cells or thymocyte membranes
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunoprecipitation – Reported in literature 1
ELISA – Reported in literature 3
Cytoxicity – Reported in literature 2
Blocking – Reported in literature 4

RRID Number AB_2794869
Gene ID 12507 (Mouse)
Gene ID Symbol Cd5 (Mouse)
Gene ID Aliases Ly-1; Ly-12; Ly-A; Lyt-1
UniProt ID P13379 (Mouse)
UniProt Name CD5_MOUSE (Mouse)

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  1. 1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, IP, FC)
  2. 2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
  3. 3. Luo W, van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992;148:1630-4. (ELISA)
  4. 4. Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S. A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5. Int Immunol. 1998;10:1185-96. (Block)