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  • Rabbit F(ab')2 IgG-UNLB

Rabbit F(ab')2 IgG-UNLB

Cat. No.:
0112-01
Purified Rabbit F(ab')2 IgG for use as a control in flow cytometry assays and as a standard in ELISA.
$213.00
Size Price (USD) Quantity
5.0 mg $213.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit F(ab')2 IgG
Source Pepsin digest of Rabbit IgG (SB Cat. No. 0111)
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
Flow Cytometry
ELISA
FLISA

RRID Number AB_2793968

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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  1. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  2. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat F(ab')2 IgG-UNLB
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Normal Rabbit Serum
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  5. Human peripheral blood lymphocytes were stained with Mouse F(ab')<sub>2</sub> IgG<sub>1</sub>-UNLB (SB Cat. No. 0115-01; gray) and Mouse F(ab')<sub>2</sub> Anti-Human CD19-UNLB (SB Cat. No. 9342-01) followed by Goat Anti-Mouse IgG(H+L), Human ads-FITC (SB Cat. No. 1031-02).
    Mouse F(ab')2 IgG1-UNLB (15H6)
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    $231.00