Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Rat Anti-Human IL-10-UNLB (JES3-9D7)

Cat. No.:
Purified Anti-Human IL-10 antibody for use in flow cytometry, immunohistochemistry, ELISA, ELISpot, western blot, and multiplex assays.
Size Price (USD) Quantity
0.5 mg $220.00
More Information
Clone JES3-9D7
Isotype Rat IgG1κ
Isotype Control Rat IgG1-UNLB (KLH/G1-2-2)
Specificity Human/Viral/Rhesus/Cynomolgus/Sooty Mangabey IL-10
Alternative Names Interleukin-10, cytokine synthesis inhibitory factor, CSIF, TGIF, B-TCGF
Immunogen COS-expressed human IL-10
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA-Capture – Quality tested 1-6
ELISpot-Capture – Reported in literature 6,7
Flow Cytometry – Reported in literature 8-15
Immunohistochemistry-Frozen Sections – Reported in literature 16
Immunohistochemistry-Paraffin Sections – Reported in literature 17
Western Blot – Reported in literature 18
Neutralization – Reported in literature 1,18,19
Multiplex-Capture – Reported in literature 20

RRID Number AB_2794120
Gene ID 3586 (Human)
694931 (Rhesus)
102133450 (Cynomolgus)
105576723 (Sooty Mangabey)
Gene ID Symbol IL10 (Human)
IL10 (Rhesus)
IL10 (Cynomolgus)
IL10 (Sooty Mangabey)
Gene ID Aliases CSIF; GVHDS; IL-10; IL10A; TGIF; EGK_01722
UniProt ID P22301 (Human)
P51496 (Rhesus)
P79338 (Cynomolgus)
P46651 (Sooty Mangabey)
UniProt Name IL10_HUMAN (Human)
IL10_MACMU (Rhesus)
IL10_MACFA (Cynomolgus)
IL10_CERAT (Sooty Mangabey)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Need help with this product?
or call 800.722.2255
  1. 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
  2. 2. Gotlieb WH, Abrams JS, Watson JM, Velu TJ, Berek JS, Martínez-Maza O. Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine. 1992;4:385-90. (ELISA-Capture, Viral Reactivity)
  3. 3. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
  4. 4. Mosmann T. Measurement of mouse and human interleukin 10. Curr Protoc Immunol. 2001;6.14:1-8. (ELISA-Capture)
  5. 5. Ma Y, Thornton S, Duwel LE, Boivin GP, Giannini EH, Leiden JM, et al. Inhibition of collagen-induced arthritis in mice by viral IL-10 gene transfer. J Immunol. 1998;161:1516-24. (ELISA-Capture, Viral Reactivity)
  6. 6. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISA-Capture, ELISPOT-Capture)
  7. 7. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Capture)
  8. 8. Koshiba M, Rosin DL, Hayashi N, Linden J, Sitkovsky MV. Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999;55:614-24. (FC)
  9. 9. Kaur A, Grant RM, Means RE, McClure H, Feinberg M, Johnson RP. Diverse host responses and outcomes following simian immunodeficiency virus SIVmac239 infection in sooty mangabeys and rhesus macaques. J Virol. 1998;72:9597-611. (FC, Rhesus & Sooty Mangabey Reactivity)
  10. 10. Gauduin M, Glickman RL, Ahmad S, Yilma T, Johnson RP. Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and β-chemokine production. Proc Natl Acad Sci USA. 1999;96:14031-6. (FC, Rhesus Reactivity)
  11. 11. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (FC)
  12. 12. Gansuvd B, Hubbard WJ, Hutchings A, Thomas FT, Goodwin J, Wilson SB, et al. Phenotypic and functional characterization of long-term cultured rhesus macaque spleen-derived NKT cells. J Immunol. 2003;171:2904-11. (FC, Rhesus Reactivity)
  13. 13. Mattila JT, Diedrich CR, Lin PL, Phuah J, Flynn JL. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37. (FC, Cynomolgus Reactivity)
  14. 14. Souza-Lemos C, de-Campos SN, Teva A, Porrozzi R, Grimaldi G Jr. In situ characterization of the granulomatous immune response with time in nonhealing lesional skin of Leishmania braziliensis-infected rhesus macaques (Macaca mulatta). Vet Immunol Immunopathol. 2011;142:147-55. (FC, Rhesus Reactivity)
  15. 15. Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, et al. CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques. AIDS Res Hum Retroviruses. 2012;28:1693-702. (FC, Cynomolgus Reactivity)
  16. 16. Melgar S, Yueng MM, Bas A, Forsberg G, Suhr O, Öberg Å, et al. Over-expression of interleukin 10 in mucosal T cells of patients with active ulcerative colitis. Clin Exp Immunol. 2003;134:127-37. (IHC-FS)
  17. 17. Smith DR, Kunkel SL, Burdick MD, Wilke CA, Orringer MB, Whyte RI, et al. Production of interleukin-10 by human bronchogenic carcinoma. Am J Pathol. 1994;145:18-25. (IHC-PS)
  18. 18. Brodeur ND, Spencer JV. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity. Virus Res. 2010;153:265-8. (WB, Neut, Viral Reactivity)
  19. 19. Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (Neut)
  20. 20. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6 (Multiplex-Capture, Cynomolgus Reactivity)
See All References