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Rat Anti-Human IL-13-LE/AF (JES10-5A2)

Cat. No.:
Low Endotoxin/Azide Free Anti-Human IL-13 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, ELISA, ELISpot, and neutralization assays.
Size Price (USD) Quantity
0.5 mg $330.00
More Information
Clone JES10-5A2
Isotype Rat IgG1κ
Isotype Control Rat IgG1-LE/AF (KLH/G1-2-2)
Specificity Human IL-13
Alternative Names Interleukin-13, NC30
Immunogen COS-expressed human IL-13
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications ELISA-Capture – Quality tested 2,3
ELISA-Detection – Reported in literature 1
ELISpot-Detection – Reported in literature 4
Flow Cytometry – Reported in literature 5-10
Immunohistochemistry-Frozen Sections – Reported in literature 11-13
Immunocytochemistry – Reported in literature 14
Neutralization – Reported in literature 15

Note – May be paired with the biotinylated clone SB126d (SB Cat. No. 15930-08) in a sandwich ELISA

RRID Number AB_2794181
Gene ID 3596 (Human)
Gene ID Symbol IL13 (Human)
Gene ID Aliases IL-13; P600
UniProt ID P35225 (Human
UniProt Name IL13_HUMAN (Human)

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  1. 1. Mascart F, Verscheure V, Malfroot A, Hainaut M, Piérard D, Temerman S, et al. Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses. J Immunol. 2003;170:1504-9. (ELISA-Detection)
  2. 2. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Capture)
  3. 3. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Capture)
  4. 4. Marotti B, Rocco A, De Colibus P, Compare D, de Nucci G, Staibano S, et al. Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases. Dig Liver Dis. 2008;40:240-7. (ELISPOT-Detection)
  5. 5. Gereda JE, Leung DY, Thatayatikom A, Streib JE, Price MR, Klinnert MD, et al. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. Lancet. 2000;355:1680-3. (FC)
  6. 6. Yamamoto J, Adachi Y, Onoue Y, Adachi YS, Okabe Y, Itazawa T, et al. Differential expression of the chemokine receptors by the Th1- and Th2-type effector populations within circulating CD4+ T cells. J Leukoc Biol. 2000;68:568-74. (FC)
  7. 7. Kim CH, Kunkel EJ, Boisvert J, Johnston B, Campbell JJ, Genovese MC, et al. Bonzo/CXCR6 expression defines type 1-polarized T-cell subsets with extralymphoid tissue homing potential. J Clin Invest. 2001;107:595-601. (FC)
  8. 8. Iwasaki M, Nagata K, Takano S, Takahashi K, Ishii N, Ikezawa Z. Association of a new-type prostaglandin D2 receptor CRTH2 with circulating T helper 2 cells in patients with atopic dermatitis. J Invest Dermatol. 2002;119:609-16. (FC)
  9. 9. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (FC)
  10. 10. Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506. (FC)
  11. 11. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
  12. 12. Sparrelid E, Emanuel D, Fehniger T, Andersson U, Andersson J. Interstitial pneumonitis in bone marrow transplant recipients is associated with local production of TH2-type cytokines and lack of T cell-mediated cytotoxicity. Transplantation. 1997;63:1782-9. (IHC-FS)
  13. 13. Knörr C, Amrehn C, Seeberger H, Rosenwald A, Stilgenbauer S, Ott G, et al. Expression of costimulatory molecules in low-grade mucosa-associated lymphoid tissue-type lymphomas in vivo. Am J Pathol. 1999;155:2019-27. (IHC-FS)
  14. 14. Dakhama A, Collins ML, Ohnishi H, Goleva E, Leung DY, Alam R, et al. IL-13-producing BLT1-positive CD8 cells are increased in asthma and are associated with airway obstruction. Allergy. 2013;68:666-73. (ICC)
  15. 15. Punnonen J, Yssel H, de Vries JE. The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells. J Allergy Clin Immunol. 1997;100:792-801. (Neut)
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