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Rat Anti-Mouse CD122-LE/AF (5H4)
Cat. No.:
1905-14
Low Endotoxin/Azide Free Anti-Mouse CD122 antibody for use in flow cytometry and immunoprecipitation assays.
$270.00
| Clone | 5H4 |
|---|---|
| Isotype | Rat (Lewis) IgG2aκ |
| Isotype Control | Rat IgG2a-LE/AF (KLH/G2a-1-1) |
| Specificity | Mouse CD122 |
| Alternative Names | IL-2Rβ, IL-15 receptor β chain |
| Description | The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the α chain which binds IL-2 with low affinity; (ii) the β chain that binds IL-2 with high affinity; and (iii) the common γ chain (γc) that does not bind IL-2. The high affinity receptor complex is an α/β/γ heterotrimer with a Kd of 1.3 x 10-11 M. In mouse spleen, CD122 is expresed on ~30% of CD8+ cells and all NK cells but <1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8- double negative cells. Cytoplasmic regions of the IL-2R β chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes. The monoclonal antibody 5H4 does not block IL-2 binding. |
| Immunogen | Rat myeloma YB2/0 transfected with truncated IL-2Rβ cDNA (YB2/0-mβt-28) |
| Conjugate | LE/AF (Low Endotoxin/Azide Free) |
| Buffer Formulation | Phosphate buffered saline, pH 7.4 |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Handle under aseptic conditions |
| Applications |
Flow Cytometry – Quality tested 1,2 Immunoprecipitation – Reported in literature 1,2 |
| RRID Number | AB_2795484 |
| Gene ID |
16185 (Mouse) |
| Gene ID Symbol |
Il2rb (Mouse) |
| Gene ID Aliases | p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta |
| UniProt ID |
P16297 (Mouse |
| UniProt Name |
IL2RB_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Malek TR, Furse RK, Fleming ML, Fadell AJ, He Y. Biochemical identity and characterization of the mouse interleukin-2 receptor β and γc subunits. J Interferon Cytokine Res. 1995;15:447-54. (Immunogen, FC, IP)
- 2. Furse RK, Malek TR. Selection of internalization-deficient cells by interleukin-2-Pseudomonas exotoxin chimeric protein: the cytoplasmic domain of the interleukin-2 receptor β chain does not contribute to internalization of interleukin-2. Eur J Immunol. 1993;23:3181-8. (FC, IP)
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