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Mouse Anti-Human CD25-AF647 (BC96)

Cat. No.:
9582-31
Alexa Fluor® 647 Anti-Human CD25 antibody for use in flow cytometry and immunohistochemistry assays.
$253.00
Size Price (USD) Quantity
100 tests $253.00
More Information
Clone BC96
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-AF647 (15H6)
Specificity Human/Rhesus/Cynomolgus CD25
Alternative Names IL-2Rα, Tac antigen
Description The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. CD25 is expressed on activated B cells, monocytes/macrophages, and T cells.
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 2,3
Immunohistochemistry-Frozen Sections – Reported in literature 1

RRID Number AB_2796980
Gene ID 3559 (Human)
574300 (Rhesus)
102123605 (Cynomolgus)
Gene ID Symbol IL2RA (Human)
IL2RA (Rhesus)
IL2RA (Cynomolgus)
Gene ID Aliases p55; CD25; IL2R; IMD41; TCGFR; IDDM10; IL-2RA
UniProt ID P01589 (Human)
Q5MNY4 (Rhesus)
UniProt Name IL2RA_HUMAN (Human)
IL2RA_MACMU (Rhesus)

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  1. 1. Ernst CW, Lee JE, Nakanishi T, Karimbux NY, Rezende TM, Stashenko P, et al. Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease. Clin Exp Immunol. 2007;148:271-80. (IHC-FS)
  2. 2. Cornwell WD, Lewis MG, Fan X, Rappaport J, Rogers TJ. Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques. J Neuroimmunol. 2013;265:43-50. (FC, Rhesus Reactivity)
  3. 3. Willer DO, Ambagala AP, Pilon R, Chan JK, Fournier J, Brooks J, et al. Experimental infection of Cynomolgus Macaques (Macaca fascicularis) with human varicella-zoster virus. J Virol. 2012;86:3626-34. (FC, Cynomolgus Reactivity)